Figure 1. Immunoprecipitation using the Diagenode antibody directed against ELAVL2
Immunoprecipitation was performed on total RNA isolated from 10 million 293T cells transfected with an ELAVL2 expression vector using 15 µg of the Diagenode antibody against ELAVL2 (Cat. No. C15410347) or with an equal amount of rabbit IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the ELAVL2 antibody (upper right). The lower figure shows the gel image for the negative IgG control, the ELAVL2 antibody, and the input (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.