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Polyclonal antibody raised in rabbit against mouse BMI1 (BMI1 polycomb ring finger oncogene) using two KLH-conjugated synthetic peptides containing a sequence from the central and the C-terminal region of the protein, respectively.
This product is for research use only. Not for use in diagnostic or therapeutic procedures.
* The optimal dilution for other applications should be determined by the end user. For WB we suggest starting with a 1:500 dilution.
Figure 1. Determination of the antibody titer To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against mouse BMI1 (cat. No. CS-PA021-100). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:3,100.