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Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against AsCRISPR/Cpf1 Western blot was performed on protein extracts from HEK293 cells transfected with HA-tagged AsCRISPR/Cpf1 (lane 1, 3) or HEK293 cells transfected with HA-tagged LbCRISPR/Cpf1 (lane 2, 4) using the Diagenode monoclonal antibody against AsCRISPR/Cpf1 (cat. No. C15200234), diluted 1:1,000 in PBS-T containing 0.5% NFDM (lane 1 and 2). Lanes 3 and 4 show the WB result with an antibody against the HA-tag.
WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.
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