We are proud to offer the first antibodies specifically recognizing the Cpf1 nuclease, described as a potential candidate for genome editing in mammalian cells.
Western blot was performed on protein extracts from HEK293 cells using the Diagenode antibody against AsCRISPR/Cpf1 (C15310262), diluted 1:5,000 in PBS-T containing 3% NFDM. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right. Lane 1 shows the Western blot analysis with the pre-immune serum, used as a negative control.
Transiently transfected HEK293 cells expressing HA-tagged LbCRISPR/Cpf1 were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the LbCRISPR/Cpf1 antibody (Cat. No. C15310263) diluted 1:500 in blocking solution at 4°C o/n followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-HA antibody.
Acidaminococcus sp. CRISPR/Cpf1 polyclonal antibody
|WB||Whole antiserum from rabbit containing 0.05% azide.|
L. bacterium CRISPR/Cpf1 monoclonal antibody
(50 µg/50 µl)
|WB||Protein G purified monoclonal antibody in TBS containing 0.02 % Na-azide.|
L. bacterium CRISPR/Cpf1 polyclonal antibody
|IF IP WB||Whole antiserum from rabbit containing 0.05% azide.|