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Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against LbCRISPR/Cpf1 Western blot was performed on protein extracts from HEK293 cells transfected with an HA-tagged LbCRISPR/Cpf1 using the Diagenode monoclonal antibody against LbCRISPR/Cpf1 (cat. No. C15200233). The antibody was used at different dilutions. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right.
Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against LbCRISPR/Cpf1 Western blot was performed on protein extracts from HEK293 cells transfected with HA-tagged LbCRISPR/Cpf1 (lane 1), HEK293 cells transfected with HA-tagged AsCRISPR/Cpf1 (lane 2) and mock transfected HEK293 cells (lane 3) using the Diagenode monoclonal antibody against LbCRISPR/Cpf1 (cat. No. C15200233), diluted 1:1,000 in PBS-T containing 3% NFDM (left). The right figure shows a WB with an antibody against the HA-tag.
WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.
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One-step generation of modular CAR-T cells with AAV-Cpf1. Dai X, Park JJ, Du Y, Kim HR, Wang G, Errami Y, Chen S Immune-cell engineering opens new capabilities for fundamental immunology research and immunotherapy. We developed a system for efficient generation of chimeric antigen receptor (CAR)-engineered T cells (CAR-T cells) with considerably enhanced features by streamlined genome engineering. By leveraging trans-activatin...