Diagenode

Optimizing DNA Shearing for Next Generation Sequencing Library Preparation with the Bioruptor®

The rapid adoption of next-generation, direct, and single- molecule sequencing technologies has made DNA shearing a critically important step in library template preparation. DNA sonication is the preferred method to prepare high quality, random, and size-appropriate sequencing libraries, and also ideal to fractionate chromatin for epigenetics studies. Here we present optimized sonication methods and protocols using the Bioruptor® sonicator to make genomic DNA libraries for the Illumina®, HeliScopeTM, SOLIDT and 454 platforms.

Related product

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    B01020001
    Bioruptor® Plus sonication device

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  • Workshop on Chromatin Proteomics
    Crete, Greece
    Oct 3-Oct 8, 2016
  • 2nd Annual Next Generation Sequencing Congress
    Boston, USA
    Oct 3-Oct 4, 2016
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