Diagenode

Next Generation Sequencing

RNA extraction

Various biochemical and analytical techniques require the extraction of RNA from tissues. Isolating intact RNA is essential for many techniques used in gene expression analysis. In order to obtain optimal yields of RNA, the efficient disruption and homogenization of tissues and cultured cells are required. Diagenode's Bioruptor® Plus uses state-of-the-art ultrasound ACT (Adaptive Cavitation Technology) combined with unique devices such as beads or tubes acting as sonication « enhancers » or dedicated reagents (such as our RNA extraction reagent) to efficiently disrupt tissues and cultured cells in just one step to deliver high quality RNA extraction.
Efficient extraction of pure RNA with high RIN. 
Total RNA profiles from mouse brain. Tissue was disrupted with Bioruptor® Plus as described in the protocol and analyzed on BioAnalyzer (Agilent). Note that small RNAs are present in all profiles indicating that the RNA is largely intact.

Made for RNA extraction

  • some alt
    C30010010-1000
    1.5 ml Bioruptor® Plus TPX microtubes
  • some alt
    C30010010-50
    1.5 ml Bioruptor® Plus TPX microtubes
  • some alt
    B01200011
    1.5 ml tube holder for Bioruptor® Standard ...
  • some alt
    C30010009
    15 ml Bioruptor® Plus TPX tubes
  • some alt
    B01200016
    15 ml sonication accessories for Bioruptor®...
  • some alt
    B01020001
    Bioruptor® Plus sonication device
  • some alt
    C20000010
    RNA Extraction kit for Bioruptor Plus

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  • 5th Annual and Final Meeting of EpiGeneSys
    Maisons des Océans, Paris, France
    Feb 11-Feb 13, 2016
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