Diagenode

STAT5aY694p polyclonal antibody - Classic

RFX5-polyclonal-antibody-diagenode
Catalog Number
Format
Price
C15410329
100 μg/ 91 μl
$295.00
  Bulk order

Alternative names: STAT5, MGF

Polyclonal antibody raised in rabbit against mouse STAT5a (Signal Transducer And Activator Of Transcription 5A) phosphorylated at Tyr694 using a KLH conjugated synthetic peptide.

Lot001
Concentration1.1 μg/μl
Species reactivityHuman, mouse, rat
TypePolyclonal
PurityAffinity purified antibody in PBS containing 0.01% sodium azide
HostRabbit
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
Western Blotting 1:1,000 Fig 1
Immunohistochemistry 1:50 Fig 2
  • Validation data

    WB

    Figure 1. Western blot analysis using the Diagenode antibody directed against STAT5aY694p
    Lysates from NK92 cells treated with IL-2 (lane 2) or untreated control cells (lane 1) were analysed by Western blot using the Diagenode antibody against STAT5aY694p (Cat. No. C15410329) diluted 1:1,000. The position of the phosphorylated STAT5a is indicated on the right, the marker (in kDa) is shown on the left. The antibody recognizes the phopsphorylated STAT5a but does not react with the unphosphorylated protein.

    IHC

    Figure 2. Immunohistochemistry using the Diagenode antibody directed against STAT5aY694p
    Formalin fixed paraffin embedded human kidney tissue was stained with the Diagenode antibody against STAT5aY694p (Cat. No. C15410329) diluted 1:50, followed by a peroxidase labelled anti-rabbit antibody.

  • Target description

    STAT5a (UniProt/Swiss-Prot entry P42229) is a member of the STAT family of transcription factors. These transcription factors are activated by phosphorylation in response to cytokines and other growth factors. Once phosphorylated the proteins are translocated from the cytoplasm to the nucleus were they activate there target genes. As such STAT proteins act as both signal transducers and transcriptional activators. STAT5 regulates the expression of milk proteins during lactation Further, STAT5 is associated with different tumors and activation of STAT5 in myeloma and lymphoma has been shown to be essential for tumorigenesis.

  • Applications
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    IHC
    Immunohistochemistry Read more
  • Documents
    STAT5aY694p polyclonal antibody - C15410329 DATASHEET
    Polyclonal antibody raised in rabbit against mouse STAT5a (Signal Transducer And Activator Of Tra...
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    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
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    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
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  • Publications

    How to properly cite this product in your work

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