Diagenode

SMAD3S423/425p polyclonal antibody - Classic

RFX5-polyclonal-antibody-diagenode
Catalog Number
Format
Price
C15410327
100 μg/ 100 μl
$295.00
  Bulk order

Alternative names: MADH3, JV15-2, HMAD-3, HSMAD3, MAD3, LDS3, HSPC193, SMAD 3

Polyclonal antibody raised in rabbit against SMAD3 (SMAD Family Member 3) phosphorylated at Ser423 and Ser425, using a KLH conjugated synthetic peptide.

Lot001
Concentration1 μg/μl
Species reactivityHuman
TypePolyclonal
PurityAffinity purified
HostRabbit
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
Western Blotting 1:2,000 Fig 1
Immunohistochemistry 1:400 Fig 2
  • Validation data

    WB

    Figure 1. Western blot analysis using the Diagenode antibody directed against SMAD3S423/425p
    Lysates from different cells treated with TGFb (+) or untreated control cells (-) were analysed by Western blot using the Diagenode antibody against SMAD3S423/425p (cat. No. C15410327) diluted 1:2,000. The position of the phosphorylated SMAD3 is indicated on the right. Figure 1 clearly shows the antibody recognizes the phopsphorylated SMAD3 whereas it has no reactivity towards the unphosphorylated SMAD3.

    IHC

    Figure 2. Immunohistochemistry using the Diagenode antibody directed against SMAD3S423/425p
    Formalin fixed paraffin embedded epidermal keratinocytes were stained with the Diagenode antibody against SMAD3S423/425p (cat. No. C15410327) diluted 1:400, followed by a peroxidise labelled anti-rabbit antibody. Figure 2 shows nuclear staining of SMAD3 (red).

  • Target description
    SMAD3 (UniProt/Swiss-Prot entry P84022) belongs to the SMAD (‘mothers against decapentaplegic) family of proteins which are
    signal transducers and transcriptional modulators involved in multiple pathways. SMAD3 is activated by TGF-beta (transforming
    growth factor) and activin type 1 receptor kinases which phosphorylates the C-terminal serines 423 and 425. SMAD3 binds
    the TRE element in the promoter region of many genes that are regulated by TGF-beta and is involved in chondrogenesis,
    osteogenesis and wound healing. It is also thought to play a role in carcinogenesis.
  • Applications
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    IHC
    Immunohistochemistry Read more
  • Documents
    SMAD3S423/425p polyclonal antibody DATASHEET
    Polyclonal antibody raised in rabbit against SMAD3 (SMAD Family Member 3) phosphorylated at Ser42...
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    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
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    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
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  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: SMAD3S423/425p polyclonal antibody - Classic (Diagenode Cat# C15410327 Lot# 001). Click here to copy to clipboard.

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