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SMAD3S423/425p polyclonal antibody

Catalog Number
100 μg/ 100 μl
  Bulk order

Alternative names: MADH3, JV15-2, HMAD-3, HSMAD3, MAD3, LDS3, HSPC193, SMAD 3

Polyclonal antibody raised in rabbit against SMAD3 (SMAD Family Member 3) phosphorylated at Ser423 and Ser425, using a KLH conjugated synthetic peptide.

Concentration1 μg/μl
Species reactivityHuman
PurityAffinity purified
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
Western Blotting 1:2,000 Fig 1
Immunohistochemistry 1:400 Fig 2
  • Validation data


    Figure 1. Western blot analysis using the Diagenode antibody directed against SMAD3S423/425p
    Lysates from different cells treated with TGFb (+) or untreated control cells (-) were analysed by Western blot using the Diagenode antibody against SMAD3S423/425p (cat. No. C15410327) diluted 1:2,000. The position of the phosphorylated SMAD3 is indicated on the right. Figure 1 clearly shows the antibody recognizes the phopsphorylated SMAD3 whereas it has no reactivity towards the unphosphorylated SMAD3.


    Figure 2. Immunohistochemistry using the Diagenode antibody directed against SMAD3S423/425p
    Formalin fixed paraffin embedded epidermal keratinocytes were stained with the Diagenode antibody against SMAD3S423/425p (cat. No. C15410327) diluted 1:400, followed by a peroxidise labelled anti-rabbit antibody. Figure 2 shows nuclear staining of SMAD3 (red).

  • Target description
    SMAD3 (UniProt/Swiss-Prot entry P84022) belongs to the SMAD (‘mothers against decapentaplegic) family of proteins which are
    signal transducers and transcriptional modulators involved in multiple pathways. SMAD3 is activated by TGF-beta (transforming
    growth factor) and activin type 1 receptor kinases which phosphorylates the C-terminal serines 423 and 425. SMAD3 binds
    the TRE element in the promoter region of many genes that are regulated by TGF-beta and is involved in chondrogenesis,
    osteogenesis and wound healing. It is also thought to play a role in carcinogenesis.
  •  Applications
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    Immunohistochemistry Read more
  •  Documents
    SMAD3S423/425p polyclonal antibody DATASHEET
    Polyclonal antibody raised in rabbit against SMAD3 (SMAD Family Member 3) phosphorylated at Ser42...
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
  •  Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: SMAD3S423/425p polyclonal antibody (Diagenode Cat# C15410327 Lot# 001). Click here to copy to clipboard.

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