Diagenode

PADI4 polyclonal antibody - Pioneer

RFX5-polyclonal-antibody-diagenode
Catalog Number
Format
Price
C15310075
(CS-075-100)
100 µl
$195.00
  Bulk order

Alternative names: PAD, HL-60 PAD, PDI4, PADI5, PDI5

Polyclonal antibody raised in rabbit against human PADI4 (Protein-arginine deiminase type-4), using a KLH-conjugated synthetic peptide containing a sequence from the N-terminus.of the protein.

LotA63-001
Concentrationnot determined
Species reactivityHuman
TypePolyclonal
PurityWhole antiserum
HostRabbit
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ELISA 1:1,000 - 1:5,000 Fig 1
Western Blotting 1:1,000 Fig 2
  • Validation Data

    ELISA

    Figure 1. Determination of the antibody titer
    To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against PADI4 (Cat. No. CS-075-100). The wells were coated with the peptide used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1: 40,200.

    Western blot

    Figure 2. Western blot analysis using the Diagenode antibody directed against PADI4
    Western blot was performed on nuclear extracts from HeLa cells (HeLa NE, 40 μg) with the Diagenode antibody against PADI4 (Cat. No. CS-075-100), diluted 1:1,000 in TBS-Tween containing 5% skimmed milk (Figure 2). The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest is indicated on the right.

  • Applications
    ELISA
    Enzyme-linked immunosorbent assay. Read more
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
  • Documents
    Datasheet PADI4 CS-075-100 DATASHEET
    Datasheet description
    Download
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
    Download
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
    Download
  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: PADI4 polyclonal antibody - Pioneer (Diagenode Cat# C15310075 Lot# A63-001 ). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

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