Diagenode

OCT4 polylconal antibody - Classic

Histone-Deacetylase-polyclonal-antibody-diagenode
Catalog Number
Format
Price
C15410305
50 μg
$295.00
  Bulk order

Polyclonal antibody raised in rabbit against OCT4 (Octamer-Binding Protein 4), using 4 KLH-conjugated synthetic peptides containing sequences from different parts of the protein.

LotA2089P
Concentration1.5 µg/µl
Species reactivityHuman
TypePolyclonal
PurityAffinity purified
HostRabbit
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ChIP * 5 μg/ChIP Fig 1, 2
ELISA 1:1,000 - 1:10,000 Fig 3
Western Blotting 1:1,000 Fig 4

* Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-10 μg per IP.

  • Validation Data

    ChIP

    Figure 1. ChIP results obtained with the Diagenode antibody directed against OCT4
    ChIP assays were performed using E14Tg2a mouse embryonic stem cells, the Diagenode antibody against OCT4 (Cat. No. C15410305) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2. 5 and 10 μg per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. Quantitative PCR was performed with primers for known OCT4 targets UTF1, YES1 and ZSCAN10, used as positive control targets, and for the promoter of the GAPDH gene, used as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

    ChIP-seq results figure A

    ChIP-seq results figure B

    ChIP-seq results figure C

    ChIP-seq results figure D

    Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against OCT4
    ChIP was performed on sheared chromatin from 4 million E14Tg2a mouse embryonic stem cells using 5 μg of the Diagenode antibody against OCT4 (Cat. No. C15410305) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 51 bp tags were aligned to the mouse genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence of mouse chromosome 7 (figure 2A) and a 1Mb region containing the UTF1 positive control (figure 2B), and in a two genomic regions surrounding the YES1 and ZSCAN10 positive control genes (figure 2C and D).

    ELISA

    Figure 3. Determination of the antibody titer
    To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against OCT4 (Cat. No. C15410305). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 2), the titer of the antibody was estimated to be 1:100,000.

    Western blot

    Figure 4. Western blot analysis using the Diagenode antibody directed against OCT4
    Nuclear extracts (15 μg) from human embryonic stem cells were analysed by Western blot using the Diagenode antibody against OCT4 (Cat. No. C15410305) diluted 1:1,000 in TBS- Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.

  • Applications
    ELISA
    Enzyme-linked immunosorbent assay. Read more
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    ChIP-seq (ab)
    Read more
    ChIP-qPCR (ab)
    Read more
  • Documents
    Datasheet OCT4 C15410305 DATASHEET
    Datasheet description
    Download
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
    Download
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
    Download
  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: OCT4 polylconal antibody - Classic (Diagenode Cat# C15410305 Lot# A2089P). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    Reversion to an embryonic alternative splicing program enhances leukemia stem cell self-renewal.
    Holm F et al.
    Formative research suggests that a human embryonic stem cell-specific alternative splicing gene regulatory network, which is repressed by Muscleblind-like (MBNL) RNA binding proteins, is involved in cell reprogramming. In this study, RNA sequencing, splice isoform-specific quantitative RT-PCR, lentiviral transductio...

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