Diagenode

Jarid1b polyclonal antibody - Pioneer

RFX5-polyclonal-antibody-diagenode
Catalog Number
Format
Price
C15310113
(CS-113-100)
100 µl
$195.00
  Bulk order

Alternative names: CT31, PLU1, PUT1, RBBP2H1, RBP2-like

Polyclonal antibody raised in rabbit against mouse Jarid1b (Jumonji, AT rich interactive domain 1B), using a KLH-conjugated synthetic peptides containing an amino acid sequence from the central part of the protein.

LotA340-004
Concentrationnot determined
Species reactivityMouse
TypePolyclonal
PurityWhole antiserum
HostRabbit
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ELISA 1:100 - 1:200 Fig 1
Western Blotting 1:500 Fig 2 (1)
  • Validation Data

    ELISA

    Figure 1. Determination of the titer
    To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against mouse Jarid1b (Cat. No. CS-113-100). The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:2,200.

    Western blot

    Figure 2. Western blot analysis using the Diagenode antibody directed against Jarid1b (1)
    Western blot was performed on whole cell lysates from mouse fibroblasts (NIH3T3) and embryonic stem cells (E14Tg2a) with the Diagenode antibody against mouse Jarid1b (Cat. No. CS-113-100), diluted 1:500 in BSA/ PBS-Tween. The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest (expected size: 179 kDa) is indicated on the right.

  • Applications
    ELISA
    Enzyme-linked immunosorbent assay. Read more
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
  • Documents
    Datasheet Jarid1b CS-113-100 DATASHEET
    Datasheet description
    Download
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
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    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
    Download
  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: Jarid1b polyclonal antibody - Pioneer (Diagenode Cat# C15310113 Lot# A340-004 ). Click here to copy to clipboard.

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