Diagenode

H4K20me2 polyclonal antibody - Classic

RFX5-polyclonal-antibody-diagenode
Catalog Number
Format
Price
C15410301
50 μg
$350.00
  Bulk order

Polyclonal antibody raised in rabbit against Histone H4 (dimethyl Lys20), using a KLH-conjugated synthetic peptide.

Lot001
Concentration0.53 μg/μl
Species reactivityHuman
TypePolyclonal
PurityAffinity purified
HostRabbit
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ChIP 2-5 μg/million cells Fig 1
Immunofluorescence 1:50 Fig 2
Western Blotting 1:2,000 Fig 3, 4
Immunochemistry 1:50
  • Validation Data

    ChIP

    Figure 1. ChIP
    Chromatin Immunoprecipitation of H4K20me2 antibody. Chromatin from one million formaldehyde cross-linked Hela cells was used with 2 μg of H4K20me2 antibody and 20 μl of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative PCR and normalized to the input chromatin.

    Immunofluorescence

    Figure 2. Immunofluorescence
    Immunofluorescence of H4K20me2 antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Primary antibody used at a 1:50 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H4K20me2 is nuclear and chromosomal. Staining: Histone H4K20me2 is expressed in green, nuclei are counterstained with DAPI (blue).

    Western Blot

    Figure 3. Western Blot
    Western Blot of H4K20me2 antibody. 30 μg C. elegans embryo lysate. Primary antibody used at a 1:2000 dilution overnight at 4°C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Predicted/Observed size: ~13 kDa. Other band(s): None.

    Western Blot

    Figure 4. Western Blot
    Western Blot of H4K20me2 antibody. 30 μg HeLa histone extracts. Primary antibody at a 1:2000 dilution overnight at 4°C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Predicted/Observed size: ~13 kDa. Other band(s): None.

  • Applications
    IF
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    ChIP-qPCR (ab)
    Read more
  • Documents
    Datasheet H4K20me2 C15410301 DATASHEET
    Datasheet description
    Download
  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: H4K20me2 polyclonal antibody - Classic (Diagenode Cat# C15410301 Lot# 001). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

  • Related products

Events

 See all events

Twitter feed

News

 See all news