Diagenode

H4K20me1 monoclonal antibody - Classic

RFX5-polyclonal-antibody-diagenode
Catalog Number
Format
Price
C15100147
(SN-147-100)
100 µl
$295.00
  Bulk order

Monoclonal antibody raised in mouse against histone H4 containing the monomethylated lysine 20 (H4K20me1), using a KLH-conjugated synthetic peptide.

Lot001
Concentrationnot determined
Species reactivityHuman
TypeMonoclonal
PurityConcentrated supernatant
HostMouse
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ChIP 3 μl/ChIP Fig 1
Dot Blotting 1:10,000 Fig 2
Western Blotting 1:1,000 Fig 3
* Please note that of the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-10 μl per IP.
  • Validation Data

    ChIP

    Figure 1 ChIP results obtained with the Diagenode monoclonal antibody directed against H4K20me1
    ChIP assays were performed using human HeLa cells, the Diagenode monclonal antibody against H4K20me1 (cat. No. SN-147-100) and optimized PCR primer sets for qPCR. ChIP was performed with the “LowCell# ChIP” kit (cat. No. kch-maglow-016), using sheared chromatin from 10,000 cells. Two different quantities of antibody (3 and 12 μl per ChIP experiment) were analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the GAPDH promoter and for the inactive gene TSH2B. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

    Cross reactivity

    Figure 2 Cross reactivity tests using the Diagenode monoclonal antibody directed against H4K20me1
    To check the specificity of the Diagenode monoclonal antibody against H4K20me1 (cat. No. SN-147-100) a Dot Blot was performed with with peptides containing different modifications or unmodified sequences of histone H4. One hundred to 0.2 pmol of peptide containing the respective histone modification were spotted on a membrane. The antibody was used at a dilution of 1:10,000. Figure 2 shows a high specificity of the antibody for the modification of interest.

    Western Blot

    Figure 3 Western blot analysis using the Diagenode monoclonal antibody directed against H4K20me1
    Histone extracts of HeLa cells (15 μg) were analysed by Western blot using the Diagenode monoclonal antibody against H4K20me1 (cat. No. SN-147-100) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.

  • Applications
    DB
    Dot blotting Read more
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    ChIP-qPCR (ab)
    Read more
  • Documents
    Datasheet H4K20me1 SN-147-100 DATASHEET
    Datasheet description
    Download
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
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    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
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  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: H4K20me1 monoclonal antibody - Classic (Diagenode Cat# C15100147 Lot# 001). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    H4K20me1 Contributes to Downregulation of X-Linked Genes for C. elegans Dosage Compensation
    Vielle A, Lang J, Dong Y, Ercan S, Kotwaliwale C, Rechtsteiner A, Appert A, Chen QB, Dose A, Egelhofer T, Kimura H, Stempor P, Dernburg A, Lieb JD, Strome S, Ahringer J
    The Caenorhabditis elegans dosage compensation complex (DCC) equalizes X-chromosome gene dosage between XO males and XX hermaphrodites by two-fold repression of X-linked gene expression in hermaphrodites. The DCC localizes to the X chromosomes in hermaphrodites but not in males, and some subunits form a complex homo...

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