Diagenode

H4K12ac monoclonal antibody - Classic (sample size)

RFX5-polyclonal-antibody-diagenode
Catalog Number
Format
Price
C15200218-10
10 μg
$80.00
  Bulk order
Other format

Monoclonal antibody raised in mouse against histone H4, aceylated at lysine 16 (H4K16ac), using a KLHconjugated synthetic peptide.

Lot001-11
Concentration1 μg/μl
Species reactivityHuman
TypeMonoclonal
PurityProtein A purified
HostMouse
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ChIP * 1-2 μg/ChIP Figure 1
Western Blotting 1:500 Figure 2

* Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μg per IP.

  • Validation Data

    ChIP

    Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against H4K12ac
    ChIP assays were performed using HeLa cells, the monoclonal antibody against H4K12ac (Cat. No. C15200218) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 1.5 million cells. A titration of the antibody consisting of 0.5, 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the coding region of the EIF2S3 gene, used as a positive control, and for the Sat2 satellite repeat, used as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

    Western Blot

    Figure 2. Western blot analysis using the Diagenode monoclonal directed antibody against H4K12ac
    Histone extracts (15 μg) from HeLa cells were analysed by Western blot using the Diagenode monoclonal antibody against H4K12ac (Cat. No. C15200218) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.

  • Applications
  • Documents
    C15200218 H4K12ac monoclonal DATASHEET
    Datasheet description
    Download
  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: H4K12ac monoclonal antibody - Classic (sample size) (Diagenode Cat# C15200218-10 Lot# 001-11). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

  • Related products

Events

  • Workshop on Chromatin Proteomics
    Crete, Greece
    Oct 3-Oct 8, 2016
  • 2nd Annual Next Generation Sequencing Congress
    Boston, USA
    Oct 3-Oct 4, 2016
  • XXXIX Reunión anual de la Sociedad de Bioquimica y Biología Molecular de Chile
    Puerto Varas, Chile
    Sep 27-Sep 30, 2016
 See all events

Twitter feed

News

 See all news