H4K12ac polyclonal antibody - Classic

Catalog Number
50 μg
  Bulk order

Polyclonal antibody raised in rabbit against Histone H4 (ac Lys12) using a KLH-conjugated synthetic peptide.

Concentration0.69 μg/μl
Species reactivityHuman
PurityAffinity purified
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
Immunofluorescence 1:100 Figure 1
Immunohistochemistry 1:100
Western Blotting 1:500 - 1:1,000 Figure 2
Dot Blotting 1:1,000 Figure 3
  • Validation Data


    Figure 1. H4K12ac Immunofluorescence results
    Immunofluorescence of H4K12ac antibody. H4K12ac antibody was tested in Hela cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).

    Western blot results

    Figure 2. H4K12ac Western blot results
    Western Blot of H4K12ac antibody. Western Blot analysis against untreated HeLa cell extracts. Load: 35 μg per lane. Primary antibody used at 1.0 μg/mL overnight at 4°C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Predicted/Observed size: 11 kDa for Histone H4K12ac antibody. Other band(s): none.

    Dot blot results

    Figure 3. H4K12ac Dot blot results
    Dot Blot of H4K12ac antibody. Lane 1: K12 unmodified. Lane 2: K12-acetylated. Load: 1, 10, and 100 picomoles of peptide. Primary antibody used at 1:1,000 for 45 min at 4°C. Secondary antibody: RABBIT IgG Secondary Antibody HRP Conjugated at 1:40,000 for 30 min at RT.

  • Applications
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
    Immunohistochemistry Read more
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    Dot blotting Read more
  • Documents
    Datasheet H4K12ac C15410302 DATASHEET
    Datasheet description
  • Publications

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