Diagenode

H3T3p monoclonal antibody - Classic

RFX5-polyclonal-antibody-diagenode
Catalog Number
Format
Price
C15210001
100 μg/100 μl
$295.00
  Bulk order

Monoclonal antibody raised in rabbit against histone H3, phosphorylated at threonine 3 (H3T3p), using a KLH-conjugated synthetic peptide.

Lot001
Concentration1 μg/μl
Species reactivityHuman
TypeMonoclonal
PurityProtein A purified
HostRabbit
StorageStore at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ChIP 0.5 - 1 μg/ChIP Fig 1
Western Blotting 1:10,000 Fig 2
Immunofluorescence 1:1,000 Fig 3
  • Validation Data

    ChIP

    Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against H3T3p
    ChIP assays were performed using HeLa cells, the Diagenode antibody against H3T3p (cat. No. C15210001) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (cat. No. C01010051), using sheared chromatin from 1 million cells. A titration consisting of 0.5, 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (1 μg/IP) was used as a negative IP control. Quantitative PCR was performed with optimized primers for the TSH2B and HBB genes, used as positive controls, and for the promoters of the EIF4A2 and GAPDH genes, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

    Western blot

    Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against H3T3p
    Histone extracts from HeLa cells (lane 1) or HeLa cells treated with nocodazole (lane 2), as well as recombinant H3 (lane 3) were analysed by Western blot using the Diagenode monoclonal antibody against H3T3p (Cat. No. C15210001) diluted 1:10,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.

    Immunofluorescence

    Figure 3. Immunofluorescence using the Diagenode monoclonal antibody directed against H3T3p
    HeLa cells were stained with the Diagenode antibody against H3T3p (Cat. No. C15210001, red) diluted 1:1,000. Actin was stained with fluorescein phalladoin (green).

  • Applications
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    IF
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
    ChIP-qPCR (ab)
    Read more
  • Documents
    H3T3p monoclonal antibody DATASHEET
    Monoclonal antibody raised in rabbit against histone H3, phosphorylated at threonine 3 (H3T3p), u...
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    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
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    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
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  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: H3T3p monoclonal antibody - Classic (Diagenode Cat# C15210001 Lot# 001). Click here to copy to clipboard.

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