Diagenode

H3K9me3 monoclonal antibody - Classic

RFX5-polyclonal-antibody-diagenode
Catalog Number
Format
Price
C15100146
(SN-146-100)
100 µl
$295.00
  Bulk order

Monoclonal antibody raised in mouse against histone H3 trimethylated at lysine 9 (H3K9me3), using a KLH-conjugated synthetic peptide.

Lot001
Concentrationnot determined
Species reactivityHuman
TypeMonoclonal
PurityConcentrated supernatant
HostMouse
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ChIP * 3 μl/ChIP Fig 1
Dot Blotting 1:10,000 Fig 2
Western Blotting 1:1,000 Fig 3
* Please note that of the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-10 μl per IP.
  • Validation Data

    ChIP

    Figure 1 ChIP results obtained with the Diagenode monoclonal antibody directed against HDAC3
    ChIP assays were performed using human HeLa cells, the Diagenode monclonal antibody against H3K9me3 (cat. No. SN-146-100) and optimized PCR primer sets for qPCR. ChIP was performed with the “LowCell# ChIP” kit (cat. No. kch-maglow-016), using sheared chromatin from 10,000 cells. Two different quantities of antibody (3 and 12 μl per ChIP experiment) were analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the GAPDH promoter and for the inactive gene TSH2B. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

    Dot Blot

    Figure 2 Cross reactivity tests using the Diagenode monoclonal antibody directed against H3K9me3
    A Dot Blot analysis was performed to test the cross reactivity of the Diagenode monoclonal antibody against H3K9me3 (cat. No. SN-146-100) with peptides containing different modifications or unmodified sequences of histone H3. One hundred to 0.2 pmol of peptide containing the respective histone modification were spotted on a membrane. The antibody was used at a dilution of 1:10,000. Figure 2 shows a high specificity of the antibody for the modification of interest.

    Western Blot

    Figure 3 Western blot analysis using the Diagenode monoclonal antibody directed against H3K9me3
    Histone extracts of HeLa cells (15 μg) were analysed by Western blot using the Diagenode monoclonal antibody against H3K9me3 (cat. No. SN-146-100) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.

  • Applications
    DB
    Dot blotting Read more
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    ChIP-qPCR (ab)
    Read more
  • Documents
    Datasheet H3K9me3 SN-146-100 DATASHEET
    Datasheet description
    Download
  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: H3K9me3 monoclonal antibody - Classic (Diagenode Cat# C15100146 Lot# 001). Click here to copy to clipboard.

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