EGFP polyclonal antibody - Classic

Catalog Number
50 µg/32 µl
  Bulk order

Polyclonal antibody raised in goat against the full length His-tagged EGFP (Enhanced Green-Fluorescent) protein

Concentration1.6 µg/µl
Species reactivityHuman
PurityAffinity purified
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
Immunofluorescence 1:800 Fig 1
  • Validation Data


    Figure 1 Immunofluorescence results obtained with the Diagenode antibody directed against EGFP
    A mitotic human HeLa cell, stably expressing a GFP-CENP-A fusion protein, was fixed with 4% formaldehyde and processed for immunofluorescence using the Diagenode antibody against EGFP (Cat. No. pAb-074-050), diluted 1:800, and a monoclonal antibody against HEC1, followed by a CY2-labeled anti-goat and a CY3-labeled anti-mouse antibody, respectively. DNA was visualized using Hoechst staining. Images of the cell were taken with a Deltavision microscope. The images shown in figure 1 represent a projection of approximately 30 0.2 μm optical sections.

  • Applications
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
  • Documents
    Datasheet EGFP pAb-074-050 DATASHEET
    Datasheet description
  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: EGFP polyclonal antibody - Classic (Diagenode Cat# C15410074 Lot# 001). Click here to copy to clipboard.

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