Figure 1 Immunofluorescence results obtained with the Diagenode antibody directed against EGFP
A mitotic human HeLa cell, stably expressing a GFP-CENP-A fusion protein, was fixed with 4% formaldehyde and processed for immunofluorescence using the Diagenode antibody against EGFP (Cat. No. pAb-074-050), diluted 1:800, and a monoclonal antibody against HEC1, followed by a CY2-labeled anti-goat and a CY3-labeled anti-mouse antibody, respectively. DNA was visualized using Hoechst staining. Images of the cell were taken with a Deltavision microscope. The images shown in figure 1 represent a projection of approximately 30 0.2 μm optical sections.