Polyclonal antibody raised in goat against the full length His-tagged EGFP (Enhanced Green-Fluorescent) protein
| Lot | 001 |
|---|---|
| Concentration | 1.6 µg/µl |
| Species reactivity | Human |
| Type | Polyclonal |
| Purity | Affinity purified |
| Host | Rabbit |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| Immunofluorescence | 1:800 | Fig 1 |
Figure 1 Immunofluorescence results obtained with the Diagenode antibody directed against EGFP
A mitotic human HeLa cell, stably expressing a GFP-CENP-A fusion protein, was fixed with 4% formaldehyde and processed for immunofluorescence using the Diagenode antibody against EGFP (Cat. No. pAb-074-050), diluted 1:800, and a monoclonal antibody against HEC1, followed by a CY2-labeled anti-goat and a CY3-labeled anti-mouse antibody, respectively. DNA was visualized using Hoechst staining. Images of the cell were taken with a Deltavision microscope. The images shown in figure 1 represent a projection of approximately 30 0.2 μm optical sections.
 How to properly cite this product in your workDiagenode strongly recommends using this: EGFP polyclonal antibody - Classic (Diagenode Cat# C15410074 Lot# 001). Click here to copy to clipboard. Using our products in your publication? Let us know! |
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