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Figure 1 Immunofluorescence results obtained with the Diagenode antibody directed against EGFP A mitotic human HeLa cell, stably expressing a GFP-CENP-A fusion protein, was fixed with 4% formaldehyde and processed for immunofluorescence using the Diagenode antibody against EGFP (Cat. No. pAb-074-050), diluted 1:800, and a monoclonal antibody against HEC1, followed by a CY2-labeled anti-goat and a CY3-labeled anti-mouse antibody, respectively. DNA was visualized using Hoechst staining. Images of the cell were taken with a Deltavision microscope. The images shown in figure 1 represent a projection of approximately 30 0.2 μm optical sections.
Diagenode offers huge selection of highly sensitive antibodies validated in IF.
Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9
HeLa cells transfected with a Cas9 expression vector (... Read more