Diagenode

Blue ladder - HRP monoclonal antibody

RFX5-polyclonal-antibody-diagenode
Catalog Number
Format
Price
C15200202-10
10 µl
$20.00
  Bulk order
Other format

The new Blue ladder - HRP monoclonal antibody allows you to develop your ladder simultaneously with your proteins of interest during western blotting. Simply add this antibody along with your secondary HRP-coupled antibody and visualize your proteins and the blue pre-stained protein ladder on one membrane.

Lot003D
Concentrationnot determined
Species reactivitySpecific for blue colour used in protein MW markers
TypeMonoclonal
PurityProtein A purified
HostMouse
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
Western Blotting 1:1,000 - 1:5,000 Fig 1, 2, 3
  • Validation data

    Figure 1. Western blot analysis using the Diagenode Blue ladder - HRP monoclonal antibody
    PAGE analysis of 5 different protein MW markers: 1. Page Ruler Plus (Thermo, cat. No. 26619); 2. Color Plus broad range (NEB, cat. No. P7711); 3. Page Ruler (Unstained, Fermentas, cat. No. SM0661); 4. Spectra Multicolor (Fermentas, cat. No. SM1859); 5. Precision Plus (Biorad, Cat. No. 161-0373). 4 μl of each marker was loaded on the gel. The right panel shows the Western blot analysis with the Diagenode Blue ladder - HRP antibody (Cat. No. C1520202) diluted 1:1,000, using a standard Western blot protocol.

    Figure 2. Western blot analysis using the Diagenode Blue ladder - HRP monoclonal antibody
    5 μl of the Page Ruler Plus Prestained Protein Ladder (Thermo Scientific, cat. No. 26619) were loaded on a gel and analyzed by Western blot with the Diagenode Blue ladder - HRP antibody (Cat. No. C1520202) used at different dilutions. Figure 2 shows that even with a 1:5,000 dilution all blue prestained bands of the molecular weight marker are clearly visible after a 30” exposure, a typical exposure time for WB analysis.

    Figure 3. Western blot analysis using the Diagenode Blue ladder - HRP monoclonal antibody
    Protein extracts from different species were subjected to SDS-PAGE and analyzed by Western blot with the Diagenode Blue ladder - HRP antibody (Cat. No. C15200202), diluted 1:1,000. The results are shown on the right. The left figure shows a Ponceau staining of the gel. This figure clearly demonstrates that the antibody does not react with any proteins of the species that were tested.

  • Applications
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
  • Documents
    Blue ladder - HRP Monoclonal Antibody DATASHEET
    Monoclonal antibody raised in mouse against the blue colour used in many prestained prote...
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  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: Blue ladder - HRP monoclonal antibody (Diagenode Cat# C15200202-10 Lot# 003D). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    Anti-RAINBOW dye-specific antibodies as universal tools for the visualization of prestained protein molecular weight markers in Western blot analysis
    Schüchner S et al.
    Western blotting is one of the most widely used techniques in molecular biology and biochemistry. Prestained proteins are used as molecular weight standards in protein electrophoresis. In the chemiluminescent Western blot analysis, however, these colored protein markers are invisible leaving researchers with the uns...

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