TET2 polyclonal antibody

Catalog Number
100 μl
  Bulk order

Polyclonal antibody raised in rabbit against TET2 (tet oncogene family member 2), using a recombinant protein.

Concentration1 μg/μl
Species reactivityhuman
PurityAffinity purified
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution * References
ChIP assay 5 μg/ChIP Fig 1
ICC/IF 1:1,000 - 1:1,000 Fig 3
Immunoprecipitation 1:100 - 1:500 Fig 2
Western Blotting 1:1,000 - 1:10,000 Fig 4

* Optimal dilutions/concentrations should be determined by the researcher.

  • Validation Data

    TET2 Antibody ChIP Grade

    Figure 1. TET2 ChIP results
    ChIP was performed with U2OS chromatin extract and 5 μg of either control rabbit IgG or TET2 antibody. The precipitated DNA was detected by PCR with primer set targeting to CCND2.

    TET2 Antibody validated in Immunoprecipitates

    Figure 2. TET2 IP results TET2 antibody immunoprecipitates TET2 protein in IP experiments. IP samples: 30 μg whole cell extract of TET2-transfected 293T cells. A. Control with 3 μg of preimmune Rabbit IgG B. Immunoprecipitation of TET2 protein by 3 μg TET2 antibody (Cat. No. C15410255) 5 % SDS-PAGE The immunoprecipitated TET2 protein was detected by TET2 antibody diluted 1:3,000.

    TET2 Antibody validated in Immunofluorescent

    Figure 3. TET2 IF results TET2 antibody detects TET2 protein in the nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TET2 protein stained by TET2 antibody (Cat. No. C15410255) diluted 1:500. Blue: Hoechst 33342 staining.

    TET2 Antibody validated in Western Blot

    Figure 4. TET2 Western blot results TET2 antibody detects TET2 protein by Western blot analysis. A. 30 μg 293T whole cell extract B. 30 μg whole cell extract of human TET2-transfected 293T cells 5 % SDS-PAGE TET2 antibody (Cat. No. C15410255) dilution: 1:5000.

  • Target description

    TET2 (UniProt/Swiss-Prot entry Q6N021) is a methylcytosine dioxygenase that catalyzes the conversion of 5-methylcytosine to 5-hydroxymethylcytosine (5-hmC). 5-hmC has been recently discovered in mammalian DNA and is abundant in Purkinje neurons, granule cells, embryonic stem cells, and brain tissue, especially in areas that are associated with higher cognitive function. Although its precise role has still to be shown, recent studies indicate that 5-hmC plays important roles distinct from 5-mC. Early evidence suggests that 5-hmC may represent a new pathway to demethylate DNA involving a repair mechanism converting 5-hmC to cytosine. Mutations in TET2 have been associated with myeloproliferative diseases such as essential thrombocythemia, polycythemia vera and primary myelofibrosis.

  •  実験手法
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
    Immunoprecipitation Read more
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    ChIP-qPCR (ab)
    Read more
  •  資料
    Datasheet TET2 C15410255 DATASHEET
    Polyclonal antibody raised in rabbit against TET2 (tet oncogene family member 2), using a recombi...
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
  •  出版物

    How to properly cite this product in your work

    Diagenode strongly recommends using this: TET2 polyclonal antibody (Diagenode Cat# C15410255-100 Lot# 41458). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    Targeting high glucose-induced epigenetic modifications at cardiac level:the role of SGLT2 and SGLT2 inhibitors.
    Scisciola L. et al.
    BACKGROUND: Sodium-glucose co-transporters (SGLT) inhibitors (SGLT2i) showed many beneficial effects at the cardiovascular level. Several mechanisms of action have been identified. However, no data on their capability to act via epigenetic mechanisms were reported. Therefore, this study aimed to investigate the abil...

    Myelodysplastic Syndrome associated TET2 mutations affect NK cellfunction and genome methylation.
    Boy M. et al.
    Myelodysplastic syndromes (MDS) are clonal hematopoietic disorders, representing high risk of progression to acute myeloid leukaemia, and frequently associated to somatic mutations, notably in the epigenetic regulator TET2. Natural Killer (NK) cells play a role in the anti-leukemic immune response via their cytolyti...

    Incretin drugs effect on epigenetic machinery: New potential therapeuticimplications in preventing vascular diabetic complications.
    Scisciola, Lucia and Rizzo, Maria Rosaria and Cataldo, Vittoria andFontanella, Rosaria Anna and Balestrieri, Maria Luisa and D'Onofrio, Nunziaand Marfella, Raffaele and Paolisso, Giuseppe and Barbieri, Michelangela
    The effect of GLP-1R agonists on DNA methylation levels of NF-κB and SOD2 genes in human aortic endothelial cells exposed to high glucose and in diabetic patients treated and not with incretin-based drugs, was evaluated. Methylation levels, mRNA and protein expression of NF-κB and SOD2 genes were measure...

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