Diagenode

5-formylcytosine (5-fC) polyclonal antibody

Catalog Number
Format
Price
C15310200
100 µl
$340.00
  Bulk order
Other format



Polyclonal antibody raised in rabbit against 5-formylcytosine (5-fC) conjugated to KLH.

LotA1941-001
Concentrationnot determined
Species reactivityHuman, mouse, other (wide range)
TypePolyclonal
PurityWhole antiserum
HostRabbit
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
DIP 3 μl/IP Fig 1
ELISA 1:10,000 Fig 2
  • Validation Data

    DIP

    Figure 1. DIP results obtained with the Diagenode antibody directed against 5-fC
    HEK293 cells were transfected with a reporter gene and hydroxymethylated in vitro with either a pCAG expression vector containing the TET2 catalytic domain (TET2cd) or a negative control pCAG vector. DIP assays were performed on 4 μg of sheared and denatured DNA using 3 μl of the Diagenode antibody against 5-fC (Cat. No. C15310200) in a total of 500 μl IP buffer. QPCR was performed with primers specific for the reporter gene. Figure 1 shows the recovery, expressed as a % of input (mean +standard deviation of 3 different experiments).

    ELISA

    Figure 2. Determination of the titer
    To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against 5-fC (Cat. No. C15310200). The plates were coated with the immunogen. By plotting the absorbance against the antibody dilution (Figure 2), the titer of the antibody was estimated to be >1:100,000.

  • Target description

    Until a few years ago, 5-methylcytosine (5-mC) was the only known modification of DNA for epigenetic regulation. In 2009, however, a second methylated cytosine, 5-hydroxymethylcytosine (5-hmC) was discovered. This new modified base is generated by enzymatic conversion of 5-mC into 5-hmC by the TET family of oxygenases.

    Recent results indicate that 5-hmC plays important roles distinct from 5-mC. Although its precise role has still to be shown, early evidence suggests that 5-hmC may well represent a new pathway to demethylate DNA involving a repair mechanism converting 5-hmC to cytosine. As such it may play a role in the regulation of gene activity. This pathway includes further oxidation of the hydroxymethyl group to a formyl or carboxyl group, both catalyzed by TET oxygenases. The formyl and carboxyl groups of 5-Formylcytosine (5-fC) and 5-Carboxylcytosine (5-caC) can be enzymatically removed without excision of the base.

    Due to their structural similarity, the different modified cytosine analogues are difficult to discriminate. The development of highly specific affinity-based reagents, such as antibodies, appears to be the most powerful way to differentially and specifically enrich 5-mC and 5-hmC sequences. We previously released highly specific antibodies directed against 5-mC, 5-hmC and 5-caC. Now, we also present a unique rabbit polyclonal antibody against 5-fC.

  •  実験手法
    ELISA
    Enzyme-linked immunosorbent assay. Read more
    DIP
    DIP Read more
  •  資料
    5-formylcytosine (5-fC) polyclonal antibody - Classic (C15310200) DATASHEET
    Polyclonal antibody raised in rabbit against 5-formylcytosine (5-fC) conjugated to KLH.
    Download
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
    Download
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
    Download
  •  Safety sheets
    5-fC antibody SDS US en Download
    5-fC antibody SDS GB en Download
    5-fC antibody SDS BE fr Download
    5-fC antibody SDS FR fr Download
    5-fC antibody SDS ES es Download
    5-fC antibody SDS DE de Download
    5-fC antibody SDS JP ja Download
    5-fC antibody SDS BE nl Download
  •  出版物

    How to properly cite this product in your work

    Diagenode strongly recommends using this: 5-formylcytosine (5-fC) polyclonal antibody (Diagenode Cat# C15310200 Lot# A1941-001). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

  •  関連商品

イベント

  • IHEC Annual meeting 2022
    Quebec, Canada
    Oct 4-Oct 7, 2022
  • Danish RNA Society's 7th Annual Meeting on RNA
    Aarhus, Denmark
    Oct 11, 2022
  • ASHG 2022
    Los Angeles, California, USA
    Oct 25-Oct 29, 2022
  • International Conference on Epigenetics and Bioengineering 2022
    Houston, Texas, USA
    Oct 27-Oct 29, 2022
  • Long-Read Sequencing Meeting Uppsala LRUA22 Meeting
    Uppsala, Sweden
    Oct 31-Nov 2, 2022
  • Association for Molecular Pathology 2022
    Phoenix, Arizona, USA
    Nov 1-Nov 5, 2022
 すべてのイベントを見る

ニュース

 すべてのニュースを見る


The European Regional Development Fund and Wallonia are investing in your future.

Extension of industrial buildings and new laboratories.


       Site map   |   Contact us   |   Conditions of sales   |   Conditions of purchase   |   Privacy policy   |   Diagenode Diagnostics