The Megaruptor® was designed to provide researchers with a simple, automated, and reproducible device for the fragmentation of DNA from 2 kb - 75 kb. Shearing performance is independent of the source, concentration, temperature, or salt content of a DNA sample. Our user-friendly software allows for two samples to be processed sequentially without additional user input and without cross-contamination. Just set the desired parameters and the automated system takes care of the rest. Clogging issues are eliminated in this design.
The Megaruptor® shows a more dense size distribution of sheared fragments, and a higher and more reproducible yield.Sylke Winkler, Sylvia Clausing, Nicola Gscheidel, Yannick Duport, Eugene Myers and Andreas Dahl, Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany, and Deep Sequencing Group, BIOTEC, TU Dresden, Germany
At the VIB Department of Molecular Genetics we strive to implement the best technologies in our workflows. Recent advances in long read sequencing using Oxford Nanopore technology have made the investigation of complex genomic variation in the human genome possible. A crucial step in the beginning of the library preparation is the shearing of genomic DNA, and this is where we implement the Megaruptor. The straightforward protocol is efficient, requires minimal sample handling and results in very reproducible fragment sizes. Another advantage is the consumable cost, which is lower than alternative technologies. For our applications we prefer shearing to lengths of 10 to 40kb, and for this the Megaruptor is the optimal solution.Wouter de Coster and the Genomic Service Facility, VIB Department of Molecular Genetics, Antwerp, Belgium