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クロマチン断片

Best Workflow Practices for
ChIP-seq Analysis

ChIPの成功に最も重要なステップは細胞の固定と溶解、そしてクロマチンの断片化の両方です。研究者の中には、これらのステップの両方の重要な性質を見過すことがあります。断片化工程の不一致を解消するDiagenodeのPicoruptor®は、最先端の超音波ACT（Adaptive Cavitation Technology）を駆使し、効率的にクロマチンを断片します。ACTは、穏やかかつ非常に有効な断片力を用いて、ChIP実験に必須の高いIP効率および感度を持つ最高のクロマチン品質を可能にします。

さらに、Picoruptor®は、タンパク質-DNA複合体が高感度で偏りのない正確なChIPのために十分に保存されるよう、熱分解からクロマチン断片を保存する正確に制御された温度環境を提供します。

Diagenode Picoruptor®は、qPCRやChIP-seqなど、最適に断片化された偏りのないクロマチンが必須な多くのアプリケーションにぴったりのクロマチン断片を実現します。

Panel A, 10 µl volume: Chromatin samples are sheared for 10, 20 and 30 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using 0.1 ml Bioruptor® Microtubes (Cat. No. B01200041). Panel B, 100 µl volume: Chromatin samples are sheared for 10 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using 0.65 ml Bioruptor® Microtubes (Cat. No. WA-005-0500). Panel C, 300 µl volume: Chromatin samples are sheared for 5, 10 and 15 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using using 1.5 ml Bioruptor microtubes (Cat. No. C30010016). Prior to de-crosslinking, samples are treated with RNase cocktail mixture at 37°C during 1 hour. The sheared chromatin is then de-crosslinked overnight and phenol/chloroform purified as described in the kit manual. 10 µl of DNA (equivalent of 500, 000 cells) are analyzed on a 2% agarose gel (MW corresponds to the 100 bp DNA molecular weight marker).

Diagenodeは、完全なクロマチンを確実にするために、SDS濃度を変えて検証されたChromatin Shearing Optimizationキットを使用することを推奨しています。

	Chromatin Shearing Kit Low SDS	Chromatin Shearing Kit Low SDS	Chromatin Shearing Kit Medium SDS	Chromatin Shearing Kit High SDS
SDS concentration	< 0.1%	0.2%	0.5%	1%
Nuclei isolation	Yes	Yes	Yes	No
Allows for shearing of... cells	100 million cells	100 million cells	100 million cells	100 million cells
Corresponding to shearing buffers from	iDeal ChIP-seq kit for Histones	iDeal ChIP-seq Kit for Transcription Factors	HighCell# ChIP kit	True MicroChIP kit LowCell# ChIP kit

Table comes from our Guide for successful chromatin preparation using the Bioruptor® Pico

クロマチン断片サービスの推奨・関連製品

B01080010

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Picoruptor 2 sonication device
B01020014

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Bioruptor® Plus sonication device
C01019027

ChIP Cross-link Gold
C01020013

Chromatin EasyShear Kit - Low SDS
C01020014

Chromatin EasyShear Kit for Plant
C01020010

Chromatin EasyShear Kit – Ultra Low SDS
B01070001

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Diagenode One sonication device
C30010015

0.1 ml Picoruptor Microtubes
B01200041

0.1 ml tube holder & tube adaptors for Picoruptor
C30010013

0.5 ml Bioruptor® Plus Microtubes
B01200043

0.5/0.65 ml tube holder for Bioruptor® Standard...
C30010011

0.65 ml Picoruptor Microtubes
C30010010-300

1.5 ml Bioruptor® Plus TPX microtubes
C30010010-50

1.5 ml Bioruptor® Plus TPX microtubes
C30010016

1.5 ml Picoruptor Microtubes with Caps
B01200011

1.5 ml tube holder for Bioruptor® Standard ...
B01200012

10 ml tube holder for Bioruptor® Standard & Plus
C01020031

15 ml Picoruptor Tubes & sonication beads
C30010009

15 ml Bioruptor® Plus TPX tubes
B01200016

15 ml sonication accessories for Picoruptor
B01200013

15 ml tube holder for Bioruptor® Standard &...
C01020012

Chromatin Shearing Optimization Kit - High SDS ...
B01201144

Tube holder for 0.2 ml tubes - Picoruptor
B01201143

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Tube holder for 0.65 ml tubes - Picoruptor
B01201140

Tube holder for 1.5 ml tubes - Picoruptor

Chromatin shearing - Guide for successful chromatin preparation using the Bioruptor^® Pico Chromatin immunoprecipitation (ChIP) is a common tool used to study protein-DNA interactions in c...	ダウンロード
Chromatin shearing from tissue protocol using Diagenode’s Chromatin shearing optimization kit - Low SDS and Bioruptor^® Standard, Plus or Pico This protocol describes the chromatin preparation from fresh or frozen tissues. The isolated chro...	ダウンロード
ChIP - The Ultimate Guide for Chromatin Shearing Optimization with Bioruptor® Standard and Plus A review of the critical points for chromatin shearing.	ダウンロード

クロマチン断片

クロマチン断片サービスの推奨・関連製品

Picoruptor 2 sonication device

Bioruptor® Plus sonication device

ChIP Cross-link Gold

Chromatin EasyShear Kit - Low SDS

Chromatin EasyShear Kit for Plant

Chromatin EasyShear Kit – Ultra Low SDS

Diagenode One sonication device

0.1 ml Picoruptor Microtubes

0.1 ml tube holder & tube adaptors for Picoruptor

0.5 ml Bioruptor® Plus Microtubes

0.5/0.65 ml tube holder for Bioruptor® Standard...

0.65 ml Picoruptor Microtubes

1.5 ml Bioruptor® Plus TPX microtubes

1.5 ml Bioruptor® Plus TPX microtubes

1.5 ml Picoruptor Microtubes with Caps

1.5 ml tube holder for Bioruptor® Standard ...

10 ml tube holder for Bioruptor® Standard & Plus

15 ml Picoruptor Tubes & sonication beads

15 ml Bioruptor® Plus TPX tubes

15 ml sonication accessories for Picoruptor

15 ml tube holder for Bioruptor® Standard &...

Chromatin Shearing Optimization Kit - High SDS ...

Tube holder for 0.2 ml tubes - Picoruptor

Tube holder for 0.65 ml tubes - Picoruptor

Tube holder for 1.5 ml tubes - Picoruptor

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