| ChIP - The Ultimate Guide for Chromatin Shearing Optimization with Bioruptor® Standard and Plus A review of the critical points for chromatin shearing. | Download |
| ChIP - The Ultimate Guide for Chromatin Shearing Optimization with Bioruptor® Standard and Plus A review of the critical points for chromatin shearing. | Download |
| Bioruptor® Standard manual MANUAL Diagenode’s Bioruptor® Standard uses a gentle method of sonication to retain the ... | Download |
| Bioruptor® Plus manual MANUAL Diagenode’s Bioruptor® Plus uses a gentle method of sonication to retain the integrity ... | Download |
 How to properly cite this product in your workDiagenode strongly recommends using this: 10 ml tube holder for Bioruptor® Standard & Plus (Diagenode Cat# B01200012). Click here to copy to clipboard. Using our products in your publication? Let us know! |
Notice (8): Undefined variable: solution_of_interest [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'jp', 'meta_keywords' => '', 'meta_description' => '10 ml tube holder for Bioruptor® Standard & Bioruptor® Plus', 'meta_title' => '10 ml tube holder for Bioruptor® Standard & Bioruptor® Plus', 'product' => array( 'Product' => array( 'id' => '1793', 'antibody_id' => null, 'name' => '10 ml tube holder for Bioruptor® Standard & Plus', 'description' => '<p><span>The attachment for 10 ml tubes has been especially designed for being used with the Bioruptor® sonication device. It allows for reproducible sonication of up to 6 x 10 ml tubes at a time.</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '1 pack', 'catalog_number' => 'B01200012', 'old_catalog_number' => 'UCD-pack 10', 'sf_code' => 'B01200012-', 'type' => 'ACC', 'search_order' => '01-Accessory', 'price_EUR' => '1275', 'price_USD' => '1627', 'price_GBP' => '1142', 'price_JPY' => '/', 'price_CNY' => '', 'price_AUD' => '4070', 'country' => 'ALL', 'except_countries' => 'Japan', 'quote' => false, 'in_stock' => true, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => '10-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack', 'meta_title' => '10 ml tube holder for Bioruptor® Standard & Bioruptor® Plus', 'meta_keywords' => '', 'meta_description' => '10 ml tube holder for Bioruptor® Standard & Bioruptor® Plus', 'modified' => '2021-03-18 12:37:01', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Slave' => array(), 'Group' => array(), 'Related' => array( (int) 0 => array( [maximum depth reached] ) ), 'Application' => array( (int) 0 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array( (int) 0 => array( [maximum depth reached] ) ), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) ) $language = 'jp' $meta_keywords = '' $meta_description = '10 ml tube holder for Bioruptor® Standard & Bioruptor® Plus' $meta_title = '10 ml tube holder for Bioruptor® Standard & Bioruptor® Plus' $product = array( 'Product' => array( 'id' => '1793', 'antibody_id' => null, 'name' => '10 ml tube holder for Bioruptor® Standard & Plus', 'description' => '<p><span>The attachment for 10 ml tubes has been especially designed for being used with the Bioruptor® sonication device. It allows for reproducible sonication of up to 6 x 10 ml tubes at a time.</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '1 pack', 'catalog_number' => 'B01200012', 'old_catalog_number' => 'UCD-pack 10', 'sf_code' => 'B01200012-', 'type' => 'ACC', 'search_order' => '01-Accessory', 'price_EUR' => '1275', 'price_USD' => '1627', 'price_GBP' => '1142', 'price_JPY' => '/', 'price_CNY' => '', 'price_AUD' => '4070', 'country' => 'ALL', 'except_countries' => 'Japan', 'quote' => false, 'in_stock' => true, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => '10-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack', 'meta_title' => '10 ml tube holder for Bioruptor® Standard & Bioruptor® Plus', 'meta_keywords' => '', 'meta_description' => '10 ml tube holder for Bioruptor® Standard & Bioruptor® Plus', 'modified' => '2021-03-18 12:37:01', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Slave' => array(), 'Group' => array(), 'Related' => array( (int) 0 => array( 'id' => '2625', 'antibody_id' => null, 'name' => '10 ml Bioruptor® Plus tubes', 'description' => '<p>This product has been discontinued. Instead, we recommend using: <a href="https://www.diagenode.com/en/p/15-ml-tpx-tubes-50-pc">15 ml Bioruptor® Plus TPX tubes</a></p> <p><span>For more information please contact </span><a href="mailto:info@diagenode.com">info@diagenode.com</a></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '100 tubes', 'catalog_number' => 'C30010012-100', 'old_catalog_number' => 'AS-100', 'sf_code' => 'C30010012-1861', 'type' => 'ACC', 'search_order' => '01-Accessory', 'price_EUR' => '/', 'price_USD' => '/', 'price_GBP' => '/', 'price_JPY' => '/', 'price_CNY' => '', 'price_AUD' => '/', 'country' => 'ALL', 'except_countries' => 'Japan', 'quote' => false, 'in_stock' => true, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => '10-ml-tubes-100-tubes', 'meta_title' => '10 ml tubes', 'meta_keywords' => '', 'meta_description' => '10 ml tubes', 'modified' => '2023-05-11 16:50:30', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ) ), 'Application' => array( (int) 0 => array( 'id' => '8', 'position' => '10', 'parent_id' => null, 'name' => 'Chromatin shearing', 'description' => '<div class="row"> <div class="small-12 medium-12 large-12 columns">The most important steps for a successful ChIP include both cell fixation and lysis, and chromatin shearing. Researchers often overlook the critical nature of both of these steps. Eliminating inconsistencies in the shearing step, <strong>Diagenode's Bioruptor</strong><sup>®</sup> uses state-of-the-art ultrasound <strong>ACT</strong> (<strong>A</strong>daptive <strong>C</strong>avitation <strong>T</strong>echnology) to efficiently shear chromatin. ACT enables the highest chromatin quality for high IP efficiency and sensitivity for ChIP experiments with gentle yet highly effective shearing forces. Additionally, the Bioruptor<sup>®</sup> provides a precisely controlled temperature environment that preserves chromatin from heat degradation such that protein-DNA complexes are well-preserved for sensitive, unbiased, and accurate ChIP.<br /><br /> <strong>Diagenode's Bioruptor</strong><sup>®</sup> is the instrument of choice for chromatin shearing used for a number of downstream applications such as qPCR and ChIP-seq that require optimally sheared, unbiased chromatin.</div> <div class="small-12 medium-12 large-12 columns text-center"><br /><img src="https://www.diagenode.com/img/applications/pico_dna_shearing_fig2.png" /></div> <div class="small-10 medium-10 large-10 columns end small-offset-1"><small> <br /><strong>Panel A, 10 µl volume:</strong> Chromatin samples are sheared for 10, 20 and 30 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using 0.1 ml Bioruptor® Microtubes (Cat. No. B01200041). <strong>Panel B, 100 µl volume:</strong> Chromatin samples are sheared for 10 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using 0.65 ml Bioruptor® Microtubes (Cat. No. WA-005-0500). <strong>Panel C, 300 µl volume:</strong> Chromatin samples are sheared for 5, 10 and 15 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using using 1.5 ml Bioruptor microtubes (Cat. No. C30010016). Prior to de-crosslinking, samples are treated with RNase cocktail mixture at 37°C during 1 hour. The sheared chromatin is then de-crosslinked overnight and phenol/chloroform purified as described in the kit manual. 10 µl of DNA (equivalent of 500, 000 cells) are analyzed on a 2% agarose gel (MW corresponds to the 100 bp DNA molecular weight marker).</small></div> <div class="small-12 medium-12 large-12 columns"><br /><br /></div> <div class="small-12 medium-12 large-12 columns"> <p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p> </div> <div class="small-12 medium-12 large-12 columns"> <div class="page" title="Page 7"> <table> <tbody> <tr valign="middle"> <td></td> <td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histone)</a></strong></td> <td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td> <td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td> <td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-medium-sds-100-million-cells">Chromatin Shearing Kit (for Plant)</a></strong></td> </tr> <tr valign="middle" style="background-color: #fff;"> <td> <p><strong>SDS concentration</strong></p> </td> <td style="text-align: center;"> <p>< 0.1%</p> </td> <td style="text-align: center;"> <p>0.2%</p> </td> <td style="text-align: center;"> <p>1%</p> </td> <td style="text-align: center;"> <p>0.5%</p> </td> </tr> <tr valign="middle" style="background-color: #fff;"> <td> <p><strong>Nuclei isolation</strong></p> </td> <td style="text-align: center;"> <p>Yes</p> </td> <td style="text-align: center;"> <p>Yes</p> </td> <td style="text-align: center;"> <p>No</p> </td> <td style="text-align: center;"> <p>Yes</p> </td> </tr> <tr valign="middle" style="background-color: #fff;"> <td> <p><strong>Allows for shearing of... cells/tissue</strong></p> </td> <td style="text-align: center;"> <p>100 million cells</p> </td> <td style="text-align: center;"> <p>100 million cells</p> </td> <td style="text-align: center;"> <p>100 million cells</p> </td> <td style="text-align: center;"> <p>up to 25 g of tissue</p> </td> </tr> <tr valign="middle" style="background-color: #fff;"> <td> <p><strong>Corresponding to shearing buffers from</strong></p> </td> <td style="text-align: center;"> <p><a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p> </td> <td style="text-align: center;"> <p><a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p> <p><a href="https://www.diagenode.com/en/p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p> </td> <td style="text-align: center;"> <p><a href="https://www.diagenode.com/en/p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p> </td> <td style="text-align: center;"> <p><a href="https://www.diagenode.com/en/p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant ChIP-seq kit</a></p> </td> </tr> </tbody> </table> <p><em><span style="font-weight: 400;">Table comes from our </span><a href="https://www.diagenode.com/protocols/bioruptor-pico-chromatin-preparation-guide"><span style="font-weight: 400;">Guide for successful chromatin preparation using the Bioruptor® Pico</span></a></em></p> </div> </div> </div>', 'in_footer' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'slug' => 'chromatin-shearing', 'meta_keywords' => 'Chromatin shearing,Chromatin Immunoprecipitation,Bioruptor,Sonication,Sonicator', 'meta_description' => 'Diagenode's Bioruptor® is the instrument of choice for chromatin shearing used for a number of downstream applications such as qPCR and ChIP-seq that require optimally sheared, unbiased chromatin.', 'meta_title' => 'Chromatin shearing using Bioruptor® sonication device | Diagenode', 'modified' => '2017-11-15 10:14:02', 'created' => '2015-03-05 15:56:30', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '67', 'position' => '1', 'parent_id' => '6', 'name' => 'Bioruptor<sup>®</sup> Plus (and Standard) accessories', 'description' => '<p><span>All you need for the smooth running of the Bioruptor</span><sup><span>®</span></sup><span> Plus (or Standard). Download our quick guide <a href="https://www.diagenode.com/documents/bioruptor-organigram-tubes">"Which tubes for which Bioruptor<sup>®</sup>?"</a><span> to find the best tubes for your applications.</span></span></p>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => false, 'all_format' => false, 'is_antibody' => false, 'slug' => 'bioruptor-plus-accessories', 'cookies_tag_id' => null, 'meta_keywords' => 'Bioruptor® Plus accessories,DNA shearing ,chromatin shearing,sonication device,sonicator', 'meta_description' => 'All you need for the smooth running of the Bioruptor® Plus', 'meta_title' => 'Accessories for Bioruptor® Plus sonication device | Diagenode', 'modified' => '2015-10-21 09:57:14', 'created' => '2015-07-23 11:39:03', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '766', 'name' => 'Bioruptor® Standard manual', 'description' => '<div class="page" title="Page 5"> <div class="layoutArea"> <div class="column"> <p><span>Diagenode’s Bioruptor</span><sup><span>® </span></sup><span>Standard uses a gentle method of sonication to retain the integrity of DNA and/or biological complexes, including chromatin, protein-protein binding, protein-DNA complexes and other biochemical and biological assay systems. </span></p> </div> </div> </div>', 'image_id' => null, 'type' => 'Manual', 'url' => 'files/products/shearing_technology/bioruptor/Bioruptor_Standard_manual.pdf', 'slug' => 'bioruptor-standard-manual', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-07-24 12:42:37', 'created' => '2015-07-07 11:47:45', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '779', 'name' => 'Bioruptor<sup>®</sup> Plus manual', 'description' => '<p><span>Diagenode’s Bioruptor</span><sup><span>® </span></sup><span>Plus uses a gentle method of sonication to retain the integrity of DNA and/or biological complexes, including chromatin, protein-protein binding, protein-DNA complexes and other biochemical and biological assay systems. </span></p>', 'image_id' => null, 'type' => 'Manual', 'url' => 'files/products/shearing_technology/bioruptor/Bioruptor_Plus_manual.pdf', 'slug' => 'bioruptor-plus-manual', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2024-06-14 15:33:56', 'created' => '2015-07-07 11:47:45', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( 'id' => '120', 'name' => 'product/shearing_technologies/B01200012_tube_holder.jpg', 'alt' => 'some alt', 'modified' => '2015-06-10 17:28:55', 'created' => '2015-06-10 17:28:55', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array( (int) 0 => array( 'id' => '18', 'name' => 'ChIP - The Ultimate Guide for Chromatin Shearing Optimization with Bioruptor® Standard and Plus', 'description' => '<p><span></span><span>A review of the critical points for chromatin shearing.</span></p>', 'image_id' => '214', 'type' => 'Protocol', 'url' => 'files/protocols/The_Ultimate_Guide_for_Chromatin_Shearing_Optimization_with_Bioruptor_protocol.pdf', 'slug' => 'the-ultimate-guide-for-chromatin-shearing-optimization-with-bioruptor-protocol', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-04-29 17:41:17', 'created' => '2015-07-20 10:35:07', 'ProductsProtocol' => array( [maximum depth reached] ) ) ), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array() $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '<li> <div class="row"> <div class="small-12 columns"> <a href="/jp/p/10-ml-tubes-100-tubes"><img src="/img/product/shearing_technologies/C30010012_tube.png" alt="some alt" class="th"/></a> </div> <div class="small-12 columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">C30010012-100</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> </div> </div> <div class="small-12 columns" > <h6 style="height:60px">10 ml Bioruptor® Plus tubes</h6> </div> </div> </li> ' $related = array( 'id' => '2625', 'antibody_id' => null, 'name' => '10 ml Bioruptor® Plus tubes', 'description' => '<p>This product has been discontinued. Instead, we recommend using: <a href="https://www.diagenode.com/en/p/15-ml-tpx-tubes-50-pc">15 ml Bioruptor® Plus TPX tubes</a></p> <p><span>For more information please contact </span><a href="mailto:info@diagenode.com">info@diagenode.com</a></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '100 tubes', 'catalog_number' => 'C30010012-100', 'old_catalog_number' => 'AS-100', 'sf_code' => 'C30010012-1861', 'type' => 'ACC', 'search_order' => '01-Accessory', 'price_EUR' => '/', 'price_USD' => '/', 'price_GBP' => '/', 'price_JPY' => '/', 'price_CNY' => '', 'price_AUD' => '/', 'country' => 'ALL', 'except_countries' => 'Japan', 'quote' => false, 'in_stock' => true, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => '10-ml-tubes-100-tubes', 'meta_title' => '10 ml tubes', 'meta_keywords' => '', 'meta_description' => '10 ml tubes', 'modified' => '2023-05-11 16:50:30', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( 'id' => '82', 'product_id' => '1793', 'related_id' => '2625' ), 'Image' => array( (int) 0 => array( 'id' => '115', 'name' => 'product/shearing_technologies/C30010012_tube.png', 'alt' => 'some alt', 'modified' => '2015-06-10 17:28:55', 'created' => '2015-06-10 17:28:55', 'ProductsImage' => array( [maximum depth reached] ) ) ) ) $rrbs_service = array( (int) 0 => (int) 1894, (int) 1 => (int) 1895 ) $chipseq_service = array( (int) 0 => (int) 2683, (int) 1 => (int) 1835, (int) 2 => (int) 1836, (int) 3 => (int) 2684, (int) 4 => (int) 1838, (int) 5 => (int) 1839, (int) 6 => (int) 1856 ) $labelize = object(Closure) { } $old_catalog_number = '<br/><small><span style="color:#CCC">(UCD-pack 10)</span></small>' $label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>' $protocol = array( 'id' => '18', 'name' => 'ChIP - The Ultimate Guide for Chromatin Shearing Optimization with Bioruptor® Standard and Plus', 'description' => '<p><span></span><span>A review of the critical points for chromatin shearing.</span></p>', 'image_id' => '214', 'type' => 'Protocol', 'url' => 'files/protocols/The_Ultimate_Guide_for_Chromatin_Shearing_Optimization_with_Bioruptor_protocol.pdf', 'slug' => 'the-ultimate-guide-for-chromatin-shearing-optimization-with-bioruptor-protocol', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-04-29 17:41:17', 'created' => '2015-07-20 10:35:07', 'ProductsProtocol' => array( 'id' => '139', 'product_id' => '1793', 'protocol_id' => '18' ) ) $document = array( 'id' => '779', 'name' => 'Bioruptor<sup>®</sup> Plus manual', 'description' => '<p><span>Diagenode’s Bioruptor</span><sup><span>® </span></sup><span>Plus uses a gentle method of sonication to retain the integrity of DNA and/or biological complexes, including chromatin, protein-protein binding, protein-DNA complexes and other biochemical and biological assay systems. </span></p>', 'image_id' => null, 'type' => 'Manual', 'url' => 'files/products/shearing_technology/bioruptor/Bioruptor_Plus_manual.pdf', 'slug' => 'bioruptor-plus-manual', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2024-06-14 15:33:56', 'created' => '2015-07-07 11:47:45', 'ProductsDocument' => array( 'id' => '102', 'product_id' => '1793', 'document_id' => '779' ) )include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118
Notice (8): Undefined variable: header [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'jp', 'meta_keywords' => '', 'meta_description' => '10 ml tube holder for Bioruptor® Standard & Bioruptor® Plus', 'meta_title' => '10 ml tube holder for Bioruptor® Standard & Bioruptor® Plus', 'product' => array( 'Product' => array( 'id' => '1793', 'antibody_id' => null, 'name' => '10 ml tube holder for Bioruptor® Standard & Plus', 'description' => '<p><span>The attachment for 10 ml tubes has been especially designed for being used with the Bioruptor® sonication device. It allows for reproducible sonication of up to 6 x 10 ml tubes at a time.</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '1 pack', 'catalog_number' => 'B01200012', 'old_catalog_number' => 'UCD-pack 10', 'sf_code' => 'B01200012-', 'type' => 'ACC', 'search_order' => '01-Accessory', 'price_EUR' => '1275', 'price_USD' => '1627', 'price_GBP' => '1142', 'price_JPY' => '/', 'price_CNY' => '', 'price_AUD' => '4070', 'country' => 'ALL', 'except_countries' => 'Japan', 'quote' => false, 'in_stock' => true, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => '10-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack', 'meta_title' => '10 ml tube holder for Bioruptor® Standard & Bioruptor® Plus', 'meta_keywords' => '', 'meta_description' => '10 ml tube holder for Bioruptor® Standard & Bioruptor® Plus', 'modified' => '2021-03-18 12:37:01', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Slave' => array(), 'Group' => array(), 'Related' => array( (int) 0 => array( [maximum depth reached] ) ), 'Application' => array( (int) 0 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array( (int) 0 => array( [maximum depth reached] ) ), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) ) $language = 'jp' $meta_keywords = '' $meta_description = '10 ml tube holder for Bioruptor® Standard & Bioruptor® Plus' $meta_title = '10 ml tube holder for Bioruptor® Standard & Bioruptor® Plus' $product = array( 'Product' => array( 'id' => '1793', 'antibody_id' => null, 'name' => '10 ml tube holder for Bioruptor® Standard & Plus', 'description' => '<p><span>The attachment for 10 ml tubes has been especially designed for being used with the Bioruptor® sonication device. It allows for reproducible sonication of up to 6 x 10 ml tubes at a time.</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '1 pack', 'catalog_number' => 'B01200012', 'old_catalog_number' => 'UCD-pack 10', 'sf_code' => 'B01200012-', 'type' => 'ACC', 'search_order' => '01-Accessory', 'price_EUR' => '1275', 'price_USD' => '1627', 'price_GBP' => '1142', 'price_JPY' => '/', 'price_CNY' => '', 'price_AUD' => '4070', 'country' => 'ALL', 'except_countries' => 'Japan', 'quote' => false, 'in_stock' => true, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => '10-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack', 'meta_title' => '10 ml tube holder for Bioruptor® Standard & Bioruptor® Plus', 'meta_keywords' => '', 'meta_description' => '10 ml tube holder for Bioruptor® Standard & Bioruptor® Plus', 'modified' => '2021-03-18 12:37:01', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Slave' => array(), 'Group' => array(), 'Related' => array( (int) 0 => array( 'id' => '2625', 'antibody_id' => null, 'name' => '10 ml Bioruptor® Plus tubes', 'description' => '<p>This product has been discontinued. Instead, we recommend using: <a href="https://www.diagenode.com/en/p/15-ml-tpx-tubes-50-pc">15 ml Bioruptor® Plus TPX tubes</a></p> <p><span>For more information please contact </span><a href="mailto:info@diagenode.com">info@diagenode.com</a></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '100 tubes', 'catalog_number' => 'C30010012-100', 'old_catalog_number' => 'AS-100', 'sf_code' => 'C30010012-1861', 'type' => 'ACC', 'search_order' => '01-Accessory', 'price_EUR' => '/', 'price_USD' => '/', 'price_GBP' => '/', 'price_JPY' => '/', 'price_CNY' => '', 'price_AUD' => '/', 'country' => 'ALL', 'except_countries' => 'Japan', 'quote' => false, 'in_stock' => true, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => '10-ml-tubes-100-tubes', 'meta_title' => '10 ml tubes', 'meta_keywords' => '', 'meta_description' => '10 ml tubes', 'modified' => '2023-05-11 16:50:30', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ) ), 'Application' => array( (int) 0 => array( 'id' => '8', 'position' => '10', 'parent_id' => null, 'name' => 'Chromatin shearing', 'description' => '<div class="row"> <div class="small-12 medium-12 large-12 columns">The most important steps for a successful ChIP include both cell fixation and lysis, and chromatin shearing. Researchers often overlook the critical nature of both of these steps. Eliminating inconsistencies in the shearing step, <strong>Diagenode's Bioruptor</strong><sup>®</sup> uses state-of-the-art ultrasound <strong>ACT</strong> (<strong>A</strong>daptive <strong>C</strong>avitation <strong>T</strong>echnology) to efficiently shear chromatin. ACT enables the highest chromatin quality for high IP efficiency and sensitivity for ChIP experiments with gentle yet highly effective shearing forces. Additionally, the Bioruptor<sup>®</sup> provides a precisely controlled temperature environment that preserves chromatin from heat degradation such that protein-DNA complexes are well-preserved for sensitive, unbiased, and accurate ChIP.<br /><br /> <strong>Diagenode's Bioruptor</strong><sup>®</sup> is the instrument of choice for chromatin shearing used for a number of downstream applications such as qPCR and ChIP-seq that require optimally sheared, unbiased chromatin.</div> <div class="small-12 medium-12 large-12 columns text-center"><br /><img src="https://www.diagenode.com/img/applications/pico_dna_shearing_fig2.png" /></div> <div class="small-10 medium-10 large-10 columns end small-offset-1"><small> <br /><strong>Panel A, 10 µl volume:</strong> Chromatin samples are sheared for 10, 20 and 30 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using 0.1 ml Bioruptor® Microtubes (Cat. No. B01200041). <strong>Panel B, 100 µl volume:</strong> Chromatin samples are sheared for 10 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using 0.65 ml Bioruptor® Microtubes (Cat. No. WA-005-0500). <strong>Panel C, 300 µl volume:</strong> Chromatin samples are sheared for 5, 10 and 15 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using using 1.5 ml Bioruptor microtubes (Cat. No. C30010016). Prior to de-crosslinking, samples are treated with RNase cocktail mixture at 37°C during 1 hour. The sheared chromatin is then de-crosslinked overnight and phenol/chloroform purified as described in the kit manual. 10 µl of DNA (equivalent of 500, 000 cells) are analyzed on a 2% agarose gel (MW corresponds to the 100 bp DNA molecular weight marker).</small></div> <div class="small-12 medium-12 large-12 columns"><br /><br /></div> <div class="small-12 medium-12 large-12 columns"> <p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p> </div> <div class="small-12 medium-12 large-12 columns"> <div class="page" title="Page 7"> <table> <tbody> <tr valign="middle"> <td></td> <td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histone)</a></strong></td> <td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td> <td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td> <td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-medium-sds-100-million-cells">Chromatin Shearing Kit (for Plant)</a></strong></td> </tr> <tr valign="middle" style="background-color: #fff;"> <td> <p><strong>SDS concentration</strong></p> </td> <td style="text-align: center;"> <p>< 0.1%</p> </td> <td style="text-align: center;"> <p>0.2%</p> </td> <td style="text-align: center;"> <p>1%</p> </td> <td style="text-align: center;"> <p>0.5%</p> </td> </tr> <tr valign="middle" style="background-color: #fff;"> <td> <p><strong>Nuclei isolation</strong></p> </td> <td style="text-align: center;"> <p>Yes</p> </td> <td style="text-align: center;"> <p>Yes</p> </td> <td style="text-align: center;"> <p>No</p> </td> <td style="text-align: center;"> <p>Yes</p> </td> </tr> <tr valign="middle" style="background-color: #fff;"> <td> <p><strong>Allows for shearing of... cells/tissue</strong></p> </td> <td style="text-align: center;"> <p>100 million cells</p> </td> <td style="text-align: center;"> <p>100 million cells</p> </td> <td style="text-align: center;"> <p>100 million cells</p> </td> <td style="text-align: center;"> <p>up to 25 g of tissue</p> </td> </tr> <tr valign="middle" style="background-color: #fff;"> <td> <p><strong>Corresponding to shearing buffers from</strong></p> </td> <td style="text-align: center;"> <p><a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p> </td> <td style="text-align: center;"> <p><a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p> <p><a href="https://www.diagenode.com/en/p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p> </td> <td style="text-align: center;"> <p><a href="https://www.diagenode.com/en/p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p> </td> <td style="text-align: center;"> <p><a href="https://www.diagenode.com/en/p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant ChIP-seq kit</a></p> </td> </tr> </tbody> </table> <p><em><span style="font-weight: 400;">Table comes from our </span><a href="https://www.diagenode.com/protocols/bioruptor-pico-chromatin-preparation-guide"><span style="font-weight: 400;">Guide for successful chromatin preparation using the Bioruptor® Pico</span></a></em></p> </div> </div> </div>', 'in_footer' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'slug' => 'chromatin-shearing', 'meta_keywords' => 'Chromatin shearing,Chromatin Immunoprecipitation,Bioruptor,Sonication,Sonicator', 'meta_description' => 'Diagenode's Bioruptor® is the instrument of choice for chromatin shearing used for a number of downstream applications such as qPCR and ChIP-seq that require optimally sheared, unbiased chromatin.', 'meta_title' => 'Chromatin shearing using Bioruptor® sonication device | Diagenode', 'modified' => '2017-11-15 10:14:02', 'created' => '2015-03-05 15:56:30', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '67', 'position' => '1', 'parent_id' => '6', 'name' => 'Bioruptor<sup>®</sup> Plus (and Standard) accessories', 'description' => '<p><span>All you need for the smooth running of the Bioruptor</span><sup><span>®</span></sup><span> Plus (or Standard). Download our quick guide <a href="https://www.diagenode.com/documents/bioruptor-organigram-tubes">"Which tubes for which Bioruptor<sup>®</sup>?"</a><span> to find the best tubes for your applications.</span></span></p>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => false, 'all_format' => false, 'is_antibody' => false, 'slug' => 'bioruptor-plus-accessories', 'cookies_tag_id' => null, 'meta_keywords' => 'Bioruptor® Plus accessories,DNA shearing ,chromatin shearing,sonication device,sonicator', 'meta_description' => 'All you need for the smooth running of the Bioruptor® Plus', 'meta_title' => 'Accessories for Bioruptor® Plus sonication device | Diagenode', 'modified' => '2015-10-21 09:57:14', 'created' => '2015-07-23 11:39:03', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '766', 'name' => 'Bioruptor® Standard manual', 'description' => '<div class="page" title="Page 5"> <div class="layoutArea"> <div class="column"> <p><span>Diagenode’s Bioruptor</span><sup><span>® </span></sup><span>Standard uses a gentle method of sonication to retain the integrity of DNA and/or biological complexes, including chromatin, protein-protein binding, protein-DNA complexes and other biochemical and biological assay systems. </span></p> </div> </div> </div>', 'image_id' => null, 'type' => 'Manual', 'url' => 'files/products/shearing_technology/bioruptor/Bioruptor_Standard_manual.pdf', 'slug' => 'bioruptor-standard-manual', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-07-24 12:42:37', 'created' => '2015-07-07 11:47:45', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '779', 'name' => 'Bioruptor<sup>®</sup> Plus manual', 'description' => '<p><span>Diagenode’s Bioruptor</span><sup><span>® </span></sup><span>Plus uses a gentle method of sonication to retain the integrity of DNA and/or biological complexes, including chromatin, protein-protein binding, protein-DNA complexes and other biochemical and biological assay systems. </span></p>', 'image_id' => null, 'type' => 'Manual', 'url' => 'files/products/shearing_technology/bioruptor/Bioruptor_Plus_manual.pdf', 'slug' => 'bioruptor-plus-manual', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2024-06-14 15:33:56', 'created' => '2015-07-07 11:47:45', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( 'id' => '120', 'name' => 'product/shearing_technologies/B01200012_tube_holder.jpg', 'alt' => 'some alt', 'modified' => '2015-06-10 17:28:55', 'created' => '2015-06-10 17:28:55', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array( (int) 0 => array( 'id' => '18', 'name' => 'ChIP - The Ultimate Guide for Chromatin Shearing Optimization with Bioruptor® Standard and Plus', 'description' => '<p><span></span><span>A review of the critical points for chromatin shearing.</span></p>', 'image_id' => '214', 'type' => 'Protocol', 'url' => 'files/protocols/The_Ultimate_Guide_for_Chromatin_Shearing_Optimization_with_Bioruptor_protocol.pdf', 'slug' => 'the-ultimate-guide-for-chromatin-shearing-optimization-with-bioruptor-protocol', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-04-29 17:41:17', 'created' => '2015-07-20 10:35:07', 'ProductsProtocol' => array( [maximum depth reached] ) ) ), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array() $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '<li> <div class="row"> <div class="small-12 columns"> <a href="/jp/p/10-ml-tubes-100-tubes"><img src="/img/product/shearing_technologies/C30010012_tube.png" alt="some alt" class="th"/></a> </div> <div class="small-12 columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">C30010012-100</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> </div> </div> <div class="small-12 columns" > <h6 style="height:60px">10 ml Bioruptor® Plus tubes</h6> </div> </div> </li> ' $related = array( 'id' => '2625', 'antibody_id' => null, 'name' => '10 ml Bioruptor® Plus tubes', 'description' => '<p>This product has been discontinued. Instead, we recommend using: <a href="https://www.diagenode.com/en/p/15-ml-tpx-tubes-50-pc">15 ml Bioruptor® Plus TPX tubes</a></p> <p><span>For more information please contact </span><a href="mailto:info@diagenode.com">info@diagenode.com</a></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '100 tubes', 'catalog_number' => 'C30010012-100', 'old_catalog_number' => 'AS-100', 'sf_code' => 'C30010012-1861', 'type' => 'ACC', 'search_order' => '01-Accessory', 'price_EUR' => '/', 'price_USD' => '/', 'price_GBP' => '/', 'price_JPY' => '/', 'price_CNY' => '', 'price_AUD' => '/', 'country' => 'ALL', 'except_countries' => 'Japan', 'quote' => false, 'in_stock' => true, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => '10-ml-tubes-100-tubes', 'meta_title' => '10 ml tubes', 'meta_keywords' => '', 'meta_description' => '10 ml tubes', 'modified' => '2023-05-11 16:50:30', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( 'id' => '82', 'product_id' => '1793', 'related_id' => '2625' ), 'Image' => array( (int) 0 => array( 'id' => '115', 'name' => 'product/shearing_technologies/C30010012_tube.png', 'alt' => 'some alt', 'modified' => '2015-06-10 17:28:55', 'created' => '2015-06-10 17:28:55', 'ProductsImage' => array( [maximum depth reached] ) ) ) ) $rrbs_service = array( (int) 0 => (int) 1894, (int) 1 => (int) 1895 ) $chipseq_service = array( (int) 0 => (int) 2683, (int) 1 => (int) 1835, (int) 2 => (int) 1836, (int) 3 => (int) 2684, (int) 4 => (int) 1838, (int) 5 => (int) 1839, (int) 6 => (int) 1856 ) $labelize = object(Closure) { } $old_catalog_number = '<br/><small><span style="color:#CCC">(UCD-pack 10)</span></small>' $label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>' $protocol = array( 'id' => '18', 'name' => 'ChIP - The Ultimate Guide for Chromatin Shearing Optimization with Bioruptor® Standard and Plus', 'description' => '<p><span></span><span>A review of the critical points for chromatin shearing.</span></p>', 'image_id' => '214', 'type' => 'Protocol', 'url' => 'files/protocols/The_Ultimate_Guide_for_Chromatin_Shearing_Optimization_with_Bioruptor_protocol.pdf', 'slug' => 'the-ultimate-guide-for-chromatin-shearing-optimization-with-bioruptor-protocol', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-04-29 17:41:17', 'created' => '2015-07-20 10:35:07', 'ProductsProtocol' => array( 'id' => '139', 'product_id' => '1793', 'protocol_id' => '18' ) ) $document = array( 'id' => '779', 'name' => 'Bioruptor<sup>®</sup> Plus manual', 'description' => '<p><span>Diagenode’s Bioruptor</span><sup><span>® </span></sup><span>Plus uses a gentle method of sonication to retain the integrity of DNA and/or biological complexes, including chromatin, protein-protein binding, protein-DNA complexes and other biochemical and biological assay systems. </span></p>', 'image_id' => null, 'type' => 'Manual', 'url' => 'files/products/shearing_technology/bioruptor/Bioruptor_Plus_manual.pdf', 'slug' => 'bioruptor-plus-manual', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2024-06-14 15:33:56', 'created' => '2015-07-07 11:47:45', 'ProductsDocument' => array( 'id' => '102', 'product_id' => '1793', 'document_id' => '779' ) )include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118
Notice (8): Undefined variable: message [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'jp', 'meta_keywords' => '', 'meta_description' => '10 ml tube holder for Bioruptor® Standard & Bioruptor® Plus', 'meta_title' => '10 ml tube holder for Bioruptor® Standard & Bioruptor® Plus', 'product' => array( 'Product' => array( 'id' => '1793', 'antibody_id' => null, 'name' => '10 ml tube holder for Bioruptor® Standard & Plus', 'description' => '<p><span>The attachment for 10 ml tubes has been especially designed for being used with the Bioruptor® sonication device. It allows for reproducible sonication of up to 6 x 10 ml tubes at a time.</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '1 pack', 'catalog_number' => 'B01200012', 'old_catalog_number' => 'UCD-pack 10', 'sf_code' => 'B01200012-', 'type' => 'ACC', 'search_order' => '01-Accessory', 'price_EUR' => '1275', 'price_USD' => '1627', 'price_GBP' => '1142', 'price_JPY' => '/', 'price_CNY' => '', 'price_AUD' => '4070', 'country' => 'ALL', 'except_countries' => 'Japan', 'quote' => false, 'in_stock' => true, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => '10-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack', 'meta_title' => '10 ml tube holder for Bioruptor® Standard & Bioruptor® Plus', 'meta_keywords' => '', 'meta_description' => '10 ml tube holder for Bioruptor® Standard & Bioruptor® Plus', 'modified' => '2021-03-18 12:37:01', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Slave' => array(), 'Group' => array(), 'Related' => array( (int) 0 => array( [maximum depth reached] ) ), 'Application' => array( (int) 0 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array( (int) 0 => array( [maximum depth reached] ) ), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) ) $language = 'jp' $meta_keywords = '' $meta_description = '10 ml tube holder for Bioruptor® Standard & Bioruptor® Plus' $meta_title = '10 ml tube holder for Bioruptor® Standard & Bioruptor® Plus' $product = array( 'Product' => array( 'id' => '1793', 'antibody_id' => null, 'name' => '10 ml tube holder for Bioruptor® Standard & Plus', 'description' => '<p><span>The attachment for 10 ml tubes has been especially designed for being used with the Bioruptor® sonication device. It allows for reproducible sonication of up to 6 x 10 ml tubes at a time.</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '1 pack', 'catalog_number' => 'B01200012', 'old_catalog_number' => 'UCD-pack 10', 'sf_code' => 'B01200012-', 'type' => 'ACC', 'search_order' => '01-Accessory', 'price_EUR' => '1275', 'price_USD' => '1627', 'price_GBP' => '1142', 'price_JPY' => '/', 'price_CNY' => '', 'price_AUD' => '4070', 'country' => 'ALL', 'except_countries' => 'Japan', 'quote' => false, 'in_stock' => true, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => '10-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack', 'meta_title' => '10 ml tube holder for Bioruptor® Standard & Bioruptor® Plus', 'meta_keywords' => '', 'meta_description' => '10 ml tube holder for Bioruptor® Standard & Bioruptor® Plus', 'modified' => '2021-03-18 12:37:01', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Slave' => array(), 'Group' => array(), 'Related' => array( (int) 0 => array( 'id' => '2625', 'antibody_id' => null, 'name' => '10 ml Bioruptor® Plus tubes', 'description' => '<p>This product has been discontinued. Instead, we recommend using: <a href="https://www.diagenode.com/en/p/15-ml-tpx-tubes-50-pc">15 ml Bioruptor® Plus TPX tubes</a></p> <p><span>For more information please contact </span><a href="mailto:info@diagenode.com">info@diagenode.com</a></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '100 tubes', 'catalog_number' => 'C30010012-100', 'old_catalog_number' => 'AS-100', 'sf_code' => 'C30010012-1861', 'type' => 'ACC', 'search_order' => '01-Accessory', 'price_EUR' => '/', 'price_USD' => '/', 'price_GBP' => '/', 'price_JPY' => '/', 'price_CNY' => '', 'price_AUD' => '/', 'country' => 'ALL', 'except_countries' => 'Japan', 'quote' => false, 'in_stock' => true, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => '10-ml-tubes-100-tubes', 'meta_title' => '10 ml tubes', 'meta_keywords' => '', 'meta_description' => '10 ml tubes', 'modified' => '2023-05-11 16:50:30', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ) ), 'Application' => array( (int) 0 => array( 'id' => '8', 'position' => '10', 'parent_id' => null, 'name' => 'Chromatin shearing', 'description' => '<div class="row"> <div class="small-12 medium-12 large-12 columns">The most important steps for a successful ChIP include both cell fixation and lysis, and chromatin shearing. Researchers often overlook the critical nature of both of these steps. Eliminating inconsistencies in the shearing step, <strong>Diagenode's Bioruptor</strong><sup>®</sup> uses state-of-the-art ultrasound <strong>ACT</strong> (<strong>A</strong>daptive <strong>C</strong>avitation <strong>T</strong>echnology) to efficiently shear chromatin. ACT enables the highest chromatin quality for high IP efficiency and sensitivity for ChIP experiments with gentle yet highly effective shearing forces. Additionally, the Bioruptor<sup>®</sup> provides a precisely controlled temperature environment that preserves chromatin from heat degradation such that protein-DNA complexes are well-preserved for sensitive, unbiased, and accurate ChIP.<br /><br /> <strong>Diagenode's Bioruptor</strong><sup>®</sup> is the instrument of choice for chromatin shearing used for a number of downstream applications such as qPCR and ChIP-seq that require optimally sheared, unbiased chromatin.</div> <div class="small-12 medium-12 large-12 columns text-center"><br /><img src="https://www.diagenode.com/img/applications/pico_dna_shearing_fig2.png" /></div> <div class="small-10 medium-10 large-10 columns end small-offset-1"><small> <br /><strong>Panel A, 10 µl volume:</strong> Chromatin samples are sheared for 10, 20 and 30 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using 0.1 ml Bioruptor® Microtubes (Cat. No. B01200041). <strong>Panel B, 100 µl volume:</strong> Chromatin samples are sheared for 10 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using 0.65 ml Bioruptor® Microtubes (Cat. No. WA-005-0500). <strong>Panel C, 300 µl volume:</strong> Chromatin samples are sheared for 5, 10 and 15 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using using 1.5 ml Bioruptor microtubes (Cat. No. C30010016). Prior to de-crosslinking, samples are treated with RNase cocktail mixture at 37°C during 1 hour. The sheared chromatin is then de-crosslinked overnight and phenol/chloroform purified as described in the kit manual. 10 µl of DNA (equivalent of 500, 000 cells) are analyzed on a 2% agarose gel (MW corresponds to the 100 bp DNA molecular weight marker).</small></div> <div class="small-12 medium-12 large-12 columns"><br /><br /></div> <div class="small-12 medium-12 large-12 columns"> <p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p> </div> <div class="small-12 medium-12 large-12 columns"> <div class="page" title="Page 7"> <table> <tbody> <tr valign="middle"> <td></td> <td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histone)</a></strong></td> <td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td> <td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td> <td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-medium-sds-100-million-cells">Chromatin Shearing Kit (for Plant)</a></strong></td> </tr> <tr valign="middle" style="background-color: #fff;"> <td> <p><strong>SDS concentration</strong></p> </td> <td style="text-align: center;"> <p>< 0.1%</p> </td> <td style="text-align: center;"> <p>0.2%</p> </td> <td style="text-align: center;"> <p>1%</p> </td> <td style="text-align: center;"> <p>0.5%</p> </td> </tr> <tr valign="middle" style="background-color: #fff;"> <td> <p><strong>Nuclei isolation</strong></p> </td> <td style="text-align: center;"> <p>Yes</p> </td> <td style="text-align: center;"> <p>Yes</p> </td> <td style="text-align: center;"> <p>No</p> </td> <td style="text-align: center;"> <p>Yes</p> </td> </tr> <tr valign="middle" style="background-color: #fff;"> <td> <p><strong>Allows for shearing of... cells/tissue</strong></p> </td> <td style="text-align: center;"> <p>100 million cells</p> </td> <td style="text-align: center;"> <p>100 million cells</p> </td> <td style="text-align: center;"> <p>100 million cells</p> </td> <td style="text-align: center;"> <p>up to 25 g of tissue</p> </td> </tr> <tr valign="middle" style="background-color: #fff;"> <td> <p><strong>Corresponding to shearing buffers from</strong></p> </td> <td style="text-align: center;"> <p><a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p> </td> <td style="text-align: center;"> <p><a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p> <p><a href="https://www.diagenode.com/en/p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p> </td> <td style="text-align: center;"> <p><a href="https://www.diagenode.com/en/p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p> </td> <td style="text-align: center;"> <p><a href="https://www.diagenode.com/en/p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant ChIP-seq kit</a></p> </td> </tr> </tbody> </table> <p><em><span style="font-weight: 400;">Table comes from our </span><a href="https://www.diagenode.com/protocols/bioruptor-pico-chromatin-preparation-guide"><span style="font-weight: 400;">Guide for successful chromatin preparation using the Bioruptor® Pico</span></a></em></p> </div> </div> </div>', 'in_footer' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'slug' => 'chromatin-shearing', 'meta_keywords' => 'Chromatin shearing,Chromatin Immunoprecipitation,Bioruptor,Sonication,Sonicator', 'meta_description' => 'Diagenode's Bioruptor® is the instrument of choice for chromatin shearing used for a number of downstream applications such as qPCR and ChIP-seq that require optimally sheared, unbiased chromatin.', 'meta_title' => 'Chromatin shearing using Bioruptor® sonication device | Diagenode', 'modified' => '2017-11-15 10:14:02', 'created' => '2015-03-05 15:56:30', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '67', 'position' => '1', 'parent_id' => '6', 'name' => 'Bioruptor<sup>®</sup> Plus (and Standard) accessories', 'description' => '<p><span>All you need for the smooth running of the Bioruptor</span><sup><span>®</span></sup><span> Plus (or Standard). Download our quick guide <a href="https://www.diagenode.com/documents/bioruptor-organigram-tubes">"Which tubes for which Bioruptor<sup>®</sup>?"</a><span> to find the best tubes for your applications.</span></span></p>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => false, 'all_format' => false, 'is_antibody' => false, 'slug' => 'bioruptor-plus-accessories', 'cookies_tag_id' => null, 'meta_keywords' => 'Bioruptor® Plus accessories,DNA shearing ,chromatin shearing,sonication device,sonicator', 'meta_description' => 'All you need for the smooth running of the Bioruptor® Plus', 'meta_title' => 'Accessories for Bioruptor® Plus sonication device | Diagenode', 'modified' => '2015-10-21 09:57:14', 'created' => '2015-07-23 11:39:03', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '766', 'name' => 'Bioruptor® Standard manual', 'description' => '<div class="page" title="Page 5"> <div class="layoutArea"> <div class="column"> <p><span>Diagenode’s Bioruptor</span><sup><span>® </span></sup><span>Standard uses a gentle method of sonication to retain the integrity of DNA and/or biological complexes, including chromatin, protein-protein binding, protein-DNA complexes and other biochemical and biological assay systems. </span></p> </div> </div> </div>', 'image_id' => null, 'type' => 'Manual', 'url' => 'files/products/shearing_technology/bioruptor/Bioruptor_Standard_manual.pdf', 'slug' => 'bioruptor-standard-manual', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-07-24 12:42:37', 'created' => '2015-07-07 11:47:45', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '779', 'name' => 'Bioruptor<sup>®</sup> Plus manual', 'description' => '<p><span>Diagenode’s Bioruptor</span><sup><span>® </span></sup><span>Plus uses a gentle method of sonication to retain the integrity of DNA and/or biological complexes, including chromatin, protein-protein binding, protein-DNA complexes and other biochemical and biological assay systems. </span></p>', 'image_id' => null, 'type' => 'Manual', 'url' => 'files/products/shearing_technology/bioruptor/Bioruptor_Plus_manual.pdf', 'slug' => 'bioruptor-plus-manual', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2024-06-14 15:33:56', 'created' => '2015-07-07 11:47:45', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( 'id' => '120', 'name' => 'product/shearing_technologies/B01200012_tube_holder.jpg', 'alt' => 'some alt', 'modified' => '2015-06-10 17:28:55', 'created' => '2015-06-10 17:28:55', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array( (int) 0 => array( 'id' => '18', 'name' => 'ChIP - The Ultimate Guide for Chromatin Shearing Optimization with Bioruptor® Standard and Plus', 'description' => '<p><span></span><span>A review of the critical points for chromatin shearing.</span></p>', 'image_id' => '214', 'type' => 'Protocol', 'url' => 'files/protocols/The_Ultimate_Guide_for_Chromatin_Shearing_Optimization_with_Bioruptor_protocol.pdf', 'slug' => 'the-ultimate-guide-for-chromatin-shearing-optimization-with-bioruptor-protocol', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-04-29 17:41:17', 'created' => '2015-07-20 10:35:07', 'ProductsProtocol' => array( [maximum depth reached] ) ) ), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array() $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '<li> <div class="row"> <div class="small-12 columns"> <a href="/jp/p/10-ml-tubes-100-tubes"><img src="/img/product/shearing_technologies/C30010012_tube.png" alt="some alt" class="th"/></a> </div> <div class="small-12 columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">C30010012-100</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> </div> </div> <div class="small-12 columns" > <h6 style="height:60px">10 ml Bioruptor® Plus tubes</h6> </div> </div> </li> ' $related = array( 'id' => '2625', 'antibody_id' => null, 'name' => '10 ml Bioruptor® Plus tubes', 'description' => '<p>This product has been discontinued. Instead, we recommend using: <a href="https://www.diagenode.com/en/p/15-ml-tpx-tubes-50-pc">15 ml Bioruptor® Plus TPX tubes</a></p> <p><span>For more information please contact </span><a href="mailto:info@diagenode.com">info@diagenode.com</a></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '100 tubes', 'catalog_number' => 'C30010012-100', 'old_catalog_number' => 'AS-100', 'sf_code' => 'C30010012-1861', 'type' => 'ACC', 'search_order' => '01-Accessory', 'price_EUR' => '/', 'price_USD' => '/', 'price_GBP' => '/', 'price_JPY' => '/', 'price_CNY' => '', 'price_AUD' => '/', 'country' => 'ALL', 'except_countries' => 'Japan', 'quote' => false, 'in_stock' => true, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => '10-ml-tubes-100-tubes', 'meta_title' => '10 ml tubes', 'meta_keywords' => '', 'meta_description' => '10 ml tubes', 'modified' => '2023-05-11 16:50:30', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( 'id' => '82', 'product_id' => '1793', 'related_id' => '2625' ), 'Image' => array( (int) 0 => array( 'id' => '115', 'name' => 'product/shearing_technologies/C30010012_tube.png', 'alt' => 'some alt', 'modified' => '2015-06-10 17:28:55', 'created' => '2015-06-10 17:28:55', 'ProductsImage' => array( [maximum depth reached] ) ) ) ) $rrbs_service = array( (int) 0 => (int) 1894, (int) 1 => (int) 1895 ) $chipseq_service = array( (int) 0 => (int) 2683, (int) 1 => (int) 1835, (int) 2 => (int) 1836, (int) 3 => (int) 2684, (int) 4 => (int) 1838, (int) 5 => (int) 1839, (int) 6 => (int) 1856 ) $labelize = object(Closure) { } $old_catalog_number = '<br/><small><span style="color:#CCC">(UCD-pack 10)</span></small>' $label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>' $protocol = array( 'id' => '18', 'name' => 'ChIP - The Ultimate Guide for Chromatin Shearing Optimization with Bioruptor® Standard and Plus', 'description' => '<p><span></span><span>A review of the critical points for chromatin shearing.</span></p>', 'image_id' => '214', 'type' => 'Protocol', 'url' => 'files/protocols/The_Ultimate_Guide_for_Chromatin_Shearing_Optimization_with_Bioruptor_protocol.pdf', 'slug' => 'the-ultimate-guide-for-chromatin-shearing-optimization-with-bioruptor-protocol', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-04-29 17:41:17', 'created' => '2015-07-20 10:35:07', 'ProductsProtocol' => array( 'id' => '139', 'product_id' => '1793', 'protocol_id' => '18' ) ) $document = array( 'id' => '779', 'name' => 'Bioruptor<sup>®</sup> Plus manual', 'description' => '<p><span>Diagenode’s Bioruptor</span><sup><span>® </span></sup><span>Plus uses a gentle method of sonication to retain the integrity of DNA and/or biological complexes, including chromatin, protein-protein binding, protein-DNA complexes and other biochemical and biological assay systems. </span></p>', 'image_id' => null, 'type' => 'Manual', 'url' => 'files/products/shearing_technology/bioruptor/Bioruptor_Plus_manual.pdf', 'slug' => 'bioruptor-plus-manual', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2024-06-14 15:33:56', 'created' => '2015-07-07 11:47:45', 'ProductsDocument' => array( 'id' => '102', 'product_id' => '1793', 'document_id' => '779' ) )include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118
Notice (8): Undefined variable: campaign_id [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'jp', 'meta_keywords' => '', 'meta_description' => '10 ml tube holder for Bioruptor® Standard & Bioruptor® Plus', 'meta_title' => '10 ml tube holder for Bioruptor® Standard & Bioruptor® Plus', 'product' => array( 'Product' => array( 'id' => '1793', 'antibody_id' => null, 'name' => '10 ml tube holder for Bioruptor® Standard & Plus', 'description' => '<p><span>The attachment for 10 ml tubes has been especially designed for being used with the Bioruptor® sonication device. It allows for reproducible sonication of up to 6 x 10 ml tubes at a time.</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '1 pack', 'catalog_number' => 'B01200012', 'old_catalog_number' => 'UCD-pack 10', 'sf_code' => 'B01200012-', 'type' => 'ACC', 'search_order' => '01-Accessory', 'price_EUR' => '1275', 'price_USD' => '1627', 'price_GBP' => '1142', 'price_JPY' => '/', 'price_CNY' => '', 'price_AUD' => '4070', 'country' => 'ALL', 'except_countries' => 'Japan', 'quote' => false, 'in_stock' => true, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => '10-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack', 'meta_title' => '10 ml tube holder for Bioruptor® Standard & Bioruptor® Plus', 'meta_keywords' => '', 'meta_description' => '10 ml tube holder for Bioruptor® Standard & Bioruptor® Plus', 'modified' => '2021-03-18 12:37:01', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Slave' => array(), 'Group' => array(), 'Related' => array( (int) 0 => array( [maximum depth reached] ) ), 'Application' => array( (int) 0 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array( (int) 0 => array( [maximum depth reached] ) ), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) ) $language = 'jp' $meta_keywords = '' $meta_description = '10 ml tube holder for Bioruptor® Standard & Bioruptor® Plus' $meta_title = '10 ml tube holder for Bioruptor® Standard & Bioruptor® Plus' $product = array( 'Product' => array( 'id' => '1793', 'antibody_id' => null, 'name' => '10 ml tube holder for Bioruptor® Standard & Plus', 'description' => '<p><span>The attachment for 10 ml tubes has been especially designed for being used with the Bioruptor® sonication device. It allows for reproducible sonication of up to 6 x 10 ml tubes at a time.</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '1 pack', 'catalog_number' => 'B01200012', 'old_catalog_number' => 'UCD-pack 10', 'sf_code' => 'B01200012-', 'type' => 'ACC', 'search_order' => '01-Accessory', 'price_EUR' => '1275', 'price_USD' => '1627', 'price_GBP' => '1142', 'price_JPY' => '/', 'price_CNY' => '', 'price_AUD' => '4070', 'country' => 'ALL', 'except_countries' => 'Japan', 'quote' => false, 'in_stock' => true, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => '10-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack', 'meta_title' => '10 ml tube holder for Bioruptor® Standard & Bioruptor® Plus', 'meta_keywords' => '', 'meta_description' => '10 ml tube holder for Bioruptor® Standard & Bioruptor® Plus', 'modified' => '2021-03-18 12:37:01', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Slave' => array(), 'Group' => array(), 'Related' => array( (int) 0 => array( 'id' => '2625', 'antibody_id' => null, 'name' => '10 ml Bioruptor® Plus tubes', 'description' => '<p>This product has been discontinued. Instead, we recommend using: <a href="https://www.diagenode.com/en/p/15-ml-tpx-tubes-50-pc">15 ml Bioruptor® Plus TPX tubes</a></p> <p><span>For more information please contact </span><a href="mailto:info@diagenode.com">info@diagenode.com</a></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '100 tubes', 'catalog_number' => 'C30010012-100', 'old_catalog_number' => 'AS-100', 'sf_code' => 'C30010012-1861', 'type' => 'ACC', 'search_order' => '01-Accessory', 'price_EUR' => '/', 'price_USD' => '/', 'price_GBP' => '/', 'price_JPY' => '/', 'price_CNY' => '', 'price_AUD' => '/', 'country' => 'ALL', 'except_countries' => 'Japan', 'quote' => false, 'in_stock' => true, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => '10-ml-tubes-100-tubes', 'meta_title' => '10 ml tubes', 'meta_keywords' => '', 'meta_description' => '10 ml tubes', 'modified' => '2023-05-11 16:50:30', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ) ), 'Application' => array( (int) 0 => array( 'id' => '8', 'position' => '10', 'parent_id' => null, 'name' => 'Chromatin shearing', 'description' => '<div class="row"> <div class="small-12 medium-12 large-12 columns">The most important steps for a successful ChIP include both cell fixation and lysis, and chromatin shearing. Researchers often overlook the critical nature of both of these steps. Eliminating inconsistencies in the shearing step, <strong>Diagenode's Bioruptor</strong><sup>®</sup> uses state-of-the-art ultrasound <strong>ACT</strong> (<strong>A</strong>daptive <strong>C</strong>avitation <strong>T</strong>echnology) to efficiently shear chromatin. ACT enables the highest chromatin quality for high IP efficiency and sensitivity for ChIP experiments with gentle yet highly effective shearing forces. Additionally, the Bioruptor<sup>®</sup> provides a precisely controlled temperature environment that preserves chromatin from heat degradation such that protein-DNA complexes are well-preserved for sensitive, unbiased, and accurate ChIP.<br /><br /> <strong>Diagenode's Bioruptor</strong><sup>®</sup> is the instrument of choice for chromatin shearing used for a number of downstream applications such as qPCR and ChIP-seq that require optimally sheared, unbiased chromatin.</div> <div class="small-12 medium-12 large-12 columns text-center"><br /><img src="https://www.diagenode.com/img/applications/pico_dna_shearing_fig2.png" /></div> <div class="small-10 medium-10 large-10 columns end small-offset-1"><small> <br /><strong>Panel A, 10 µl volume:</strong> Chromatin samples are sheared for 10, 20 and 30 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using 0.1 ml Bioruptor® Microtubes (Cat. No. B01200041). <strong>Panel B, 100 µl volume:</strong> Chromatin samples are sheared for 10 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using 0.65 ml Bioruptor® Microtubes (Cat. No. WA-005-0500). <strong>Panel C, 300 µl volume:</strong> Chromatin samples are sheared for 5, 10 and 15 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using using 1.5 ml Bioruptor microtubes (Cat. No. C30010016). Prior to de-crosslinking, samples are treated with RNase cocktail mixture at 37°C during 1 hour. The sheared chromatin is then de-crosslinked overnight and phenol/chloroform purified as described in the kit manual. 10 µl of DNA (equivalent of 500, 000 cells) are analyzed on a 2% agarose gel (MW corresponds to the 100 bp DNA molecular weight marker).</small></div> <div class="small-12 medium-12 large-12 columns"><br /><br /></div> <div class="small-12 medium-12 large-12 columns"> <p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p> </div> <div class="small-12 medium-12 large-12 columns"> <div class="page" title="Page 7"> <table> <tbody> <tr valign="middle"> <td></td> <td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histone)</a></strong></td> <td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td> <td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td> <td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-medium-sds-100-million-cells">Chromatin Shearing Kit (for Plant)</a></strong></td> </tr> <tr valign="middle" style="background-color: #fff;"> <td> <p><strong>SDS concentration</strong></p> </td> <td style="text-align: center;"> <p>< 0.1%</p> </td> <td style="text-align: center;"> <p>0.2%</p> </td> <td style="text-align: center;"> <p>1%</p> </td> <td style="text-align: center;"> <p>0.5%</p> </td> </tr> <tr valign="middle" style="background-color: #fff;"> <td> <p><strong>Nuclei isolation</strong></p> </td> <td style="text-align: center;"> <p>Yes</p> </td> <td style="text-align: center;"> <p>Yes</p> </td> <td style="text-align: center;"> <p>No</p> </td> <td style="text-align: center;"> <p>Yes</p> </td> </tr> <tr valign="middle" style="background-color: #fff;"> <td> <p><strong>Allows for shearing of... cells/tissue</strong></p> </td> <td style="text-align: center;"> <p>100 million cells</p> </td> <td style="text-align: center;"> <p>100 million cells</p> </td> <td style="text-align: center;"> <p>100 million cells</p> </td> <td style="text-align: center;"> <p>up to 25 g of tissue</p> </td> </tr> <tr valign="middle" style="background-color: #fff;"> <td> <p><strong>Corresponding to shearing buffers from</strong></p> </td> <td style="text-align: center;"> <p><a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p> </td> <td style="text-align: center;"> <p><a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p> <p><a href="https://www.diagenode.com/en/p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p> </td> <td style="text-align: center;"> <p><a href="https://www.diagenode.com/en/p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p> </td> <td style="text-align: center;"> <p><a href="https://www.diagenode.com/en/p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant ChIP-seq kit</a></p> </td> </tr> </tbody> </table> <p><em><span style="font-weight: 400;">Table comes from our </span><a href="https://www.diagenode.com/protocols/bioruptor-pico-chromatin-preparation-guide"><span style="font-weight: 400;">Guide for successful chromatin preparation using the Bioruptor® Pico</span></a></em></p> </div> </div> </div>', 'in_footer' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'slug' => 'chromatin-shearing', 'meta_keywords' => 'Chromatin shearing,Chromatin Immunoprecipitation,Bioruptor,Sonication,Sonicator', 'meta_description' => 'Diagenode's Bioruptor® is the instrument of choice for chromatin shearing used for a number of downstream applications such as qPCR and ChIP-seq that require optimally sheared, unbiased chromatin.', 'meta_title' => 'Chromatin shearing using Bioruptor® sonication device | Diagenode', 'modified' => '2017-11-15 10:14:02', 'created' => '2015-03-05 15:56:30', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '67', 'position' => '1', 'parent_id' => '6', 'name' => 'Bioruptor<sup>®</sup> Plus (and Standard) accessories', 'description' => '<p><span>All you need for the smooth running of the Bioruptor</span><sup><span>®</span></sup><span> Plus (or Standard). Download our quick guide <a href="https://www.diagenode.com/documents/bioruptor-organigram-tubes">"Which tubes for which Bioruptor<sup>®</sup>?"</a><span> to find the best tubes for your applications.</span></span></p>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => false, 'all_format' => false, 'is_antibody' => false, 'slug' => 'bioruptor-plus-accessories', 'cookies_tag_id' => null, 'meta_keywords' => 'Bioruptor® Plus accessories,DNA shearing ,chromatin shearing,sonication device,sonicator', 'meta_description' => 'All you need for the smooth running of the Bioruptor® Plus', 'meta_title' => 'Accessories for Bioruptor® Plus sonication device | Diagenode', 'modified' => '2015-10-21 09:57:14', 'created' => '2015-07-23 11:39:03', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '766', 'name' => 'Bioruptor® Standard manual', 'description' => '<div class="page" title="Page 5"> <div class="layoutArea"> <div class="column"> <p><span>Diagenode’s Bioruptor</span><sup><span>® </span></sup><span>Standard uses a gentle method of sonication to retain the integrity of DNA and/or biological complexes, including chromatin, protein-protein binding, protein-DNA complexes and other biochemical and biological assay systems. </span></p> </div> </div> </div>', 'image_id' => null, 'type' => 'Manual', 'url' => 'files/products/shearing_technology/bioruptor/Bioruptor_Standard_manual.pdf', 'slug' => 'bioruptor-standard-manual', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-07-24 12:42:37', 'created' => '2015-07-07 11:47:45', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '779', 'name' => 'Bioruptor<sup>®</sup> Plus manual', 'description' => '<p><span>Diagenode’s Bioruptor</span><sup><span>® </span></sup><span>Plus uses a gentle method of sonication to retain the integrity of DNA and/or biological complexes, including chromatin, protein-protein binding, protein-DNA complexes and other biochemical and biological assay systems. </span></p>', 'image_id' => null, 'type' => 'Manual', 'url' => 'files/products/shearing_technology/bioruptor/Bioruptor_Plus_manual.pdf', 'slug' => 'bioruptor-plus-manual', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2024-06-14 15:33:56', 'created' => '2015-07-07 11:47:45', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( 'id' => '120', 'name' => 'product/shearing_technologies/B01200012_tube_holder.jpg', 'alt' => 'some alt', 'modified' => '2015-06-10 17:28:55', 'created' => '2015-06-10 17:28:55', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array( (int) 0 => array( 'id' => '18', 'name' => 'ChIP - The Ultimate Guide for Chromatin Shearing Optimization with Bioruptor® Standard and Plus', 'description' => '<p><span></span><span>A review of the critical points for chromatin shearing.</span></p>', 'image_id' => '214', 'type' => 'Protocol', 'url' => 'files/protocols/The_Ultimate_Guide_for_Chromatin_Shearing_Optimization_with_Bioruptor_protocol.pdf', 'slug' => 'the-ultimate-guide-for-chromatin-shearing-optimization-with-bioruptor-protocol', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-04-29 17:41:17', 'created' => '2015-07-20 10:35:07', 'ProductsProtocol' => array( [maximum depth reached] ) ) ), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array() $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '<li> <div class="row"> <div class="small-12 columns"> <a href="/jp/p/10-ml-tubes-100-tubes"><img src="/img/product/shearing_technologies/C30010012_tube.png" alt="some alt" class="th"/></a> </div> <div class="small-12 columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">C30010012-100</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> </div> </div> <div class="small-12 columns" > <h6 style="height:60px">10 ml Bioruptor® Plus tubes</h6> </div> </div> </li> ' $related = array( 'id' => '2625', 'antibody_id' => null, 'name' => '10 ml Bioruptor® Plus tubes', 'description' => '<p>This product has been discontinued. Instead, we recommend using: <a href="https://www.diagenode.com/en/p/15-ml-tpx-tubes-50-pc">15 ml Bioruptor® Plus TPX tubes</a></p> <p><span>For more information please contact </span><a href="mailto:info@diagenode.com">info@diagenode.com</a></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '100 tubes', 'catalog_number' => 'C30010012-100', 'old_catalog_number' => 'AS-100', 'sf_code' => 'C30010012-1861', 'type' => 'ACC', 'search_order' => '01-Accessory', 'price_EUR' => '/', 'price_USD' => '/', 'price_GBP' => '/', 'price_JPY' => '/', 'price_CNY' => '', 'price_AUD' => '/', 'country' => 'ALL', 'except_countries' => 'Japan', 'quote' => false, 'in_stock' => true, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => '10-ml-tubes-100-tubes', 'meta_title' => '10 ml tubes', 'meta_keywords' => '', 'meta_description' => '10 ml tubes', 'modified' => '2023-05-11 16:50:30', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( 'id' => '82', 'product_id' => '1793', 'related_id' => '2625' ), 'Image' => array( (int) 0 => array( 'id' => '115', 'name' => 'product/shearing_technologies/C30010012_tube.png', 'alt' => 'some alt', 'modified' => '2015-06-10 17:28:55', 'created' => '2015-06-10 17:28:55', 'ProductsImage' => array( [maximum depth reached] ) ) ) ) $rrbs_service = array( (int) 0 => (int) 1894, (int) 1 => (int) 1895 ) $chipseq_service = array( (int) 0 => (int) 2683, (int) 1 => (int) 1835, (int) 2 => (int) 1836, (int) 3 => (int) 2684, (int) 4 => (int) 1838, (int) 5 => (int) 1839, (int) 6 => (int) 1856 ) $labelize = object(Closure) { } $old_catalog_number = '<br/><small><span style="color:#CCC">(UCD-pack 10)</span></small>' $label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>' $protocol = array( 'id' => '18', 'name' => 'ChIP - The Ultimate Guide for Chromatin Shearing Optimization with Bioruptor® Standard and Plus', 'description' => '<p><span></span><span>A review of the critical points for chromatin shearing.</span></p>', 'image_id' => '214', 'type' => 'Protocol', 'url' => 'files/protocols/The_Ultimate_Guide_for_Chromatin_Shearing_Optimization_with_Bioruptor_protocol.pdf', 'slug' => 'the-ultimate-guide-for-chromatin-shearing-optimization-with-bioruptor-protocol', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-04-29 17:41:17', 'created' => '2015-07-20 10:35:07', 'ProductsProtocol' => array( 'id' => '139', 'product_id' => '1793', 'protocol_id' => '18' ) ) $document = array( 'id' => '779', 'name' => 'Bioruptor<sup>®</sup> Plus manual', 'description' => '<p><span>Diagenode’s Bioruptor</span><sup><span>® </span></sup><span>Plus uses a gentle method of sonication to retain the integrity of DNA and/or biological complexes, including chromatin, protein-protein binding, protein-DNA complexes and other biochemical and biological assay systems. </span></p>', 'image_id' => null, 'type' => 'Manual', 'url' => 'files/products/shearing_technology/bioruptor/Bioruptor_Plus_manual.pdf', 'slug' => 'bioruptor-plus-manual', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2024-06-14 15:33:56', 'created' => '2015-07-07 11:47:45', 'ProductsDocument' => array( 'id' => '102', 'product_id' => '1793', 'document_id' => '779' ) )include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118
To ensure you see the information most relevant to you, please select your country. Please note that your browser will need to be configured to accept cookies.
Diagenode will process your personal data in strict accordance with its privacy policy. This will include sending you updates about us, our products, and resources we think would be of interest to you.