| ChIP - The Ultimate Guide for Chromatin Shearing Optimization with Bioruptor® Standard and Plus A review of the critical points for chromatin shearing. | Download |
| ChIP - The Ultimate Guide for Chromatin Shearing Optimization with Bioruptor® Standard and Plus A review of the critical points for chromatin shearing. | Download |
| Bioruptor® Standard manual MANUAL Diagenode’s Bioruptor® Standard uses a gentle method of sonication to retain the ... | Download |
| Bioruptor® Plus manual MANUAL Diagenode’s Bioruptor® Plus uses a gentle method of sonication to retain the integrity ... | Download |
 How to properly cite this product in your workDiagenode strongly recommends using this: 10 ml tube holder for Bioruptor® Standard & Plus (Diagenode Cat# B01200012). Click here to copy to clipboard. Using our products in your publication? Let us know! |
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Researchers often overlook the critical nature of both of these steps. Eliminating inconsistencies in the shearing step, <strong>Diagenode's Bioruptor</strong><sup>®</sup> uses state-of-the-art ultrasound <strong>ACT</strong> (<strong>A</strong>daptive <strong>C</strong>avitation <strong>T</strong>echnology) to efficiently shear chromatin. ACT enables the highest chromatin quality for high IP efficiency and sensitivity for ChIP experiments with gentle yet highly effective shearing forces. Additionally, the Bioruptor<sup>®</sup> provides a precisely controlled temperature environment that preserves chromatin from heat degradation such that protein-DNA complexes are well-preserved for sensitive, unbiased, and accurate ChIP.<br /><br /> <strong>Diagenode's Bioruptor</strong><sup>®</sup> is the instrument of choice for chromatin shearing used for a number of downstream applications such as qPCR and ChIP-seq that require optimally sheared, unbiased chromatin.</div> <div class="small-12 medium-12 large-12 columns text-center"><br /><img src="https://www.diagenode.com/img/applications/pico_dna_shearing_fig2.png" /></div> <div class="small-10 medium-10 large-10 columns end small-offset-1"><small> <br /><strong>Panel A, 10 µl volume:</strong> Chromatin samples are sheared for 10, 20 and 30 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using 0.1 ml Bioruptor® Microtubes (Cat. No. B01200041). <strong>Panel B, 100 µl volume:</strong> Chromatin samples are sheared for 10 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using 0.65 ml Bioruptor® Microtubes (Cat. No. WA-005-0500). <strong>Panel C, 300 µl volume:</strong> Chromatin samples are sheared for 5, 10 and 15 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using using 1.5 ml Bioruptor microtubes (Cat. No. C30010016). Prior to de-crosslinking, samples are treated with RNase cocktail mixture at 37°C during 1 hour. The sheared chromatin is then de-crosslinked overnight and phenol/chloroform purified as described in the kit manual. 10 µl of DNA (equivalent of 500, 000 cells) are analyzed on a 2% agarose gel (MW corresponds to the 100 bp DNA molecular weight marker).</small></div> <div class="small-12 medium-12 large-12 columns"><br /><br /></div> <div class="small-12 medium-12 large-12 columns"> <p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p> </div> <div class="small-12 medium-12 large-12 columns"> <div class="page" title="Page 7"> <table> <tbody> <tr valign="middle"> <td></td> <td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histone)</a></strong></td> <td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td> <td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td> <td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-medium-sds-100-million-cells">Chromatin Shearing Kit (for Plant)</a></strong></td> </tr> <tr valign="middle" style="background-color: #fff;"> <td> <p><strong>SDS concentration</strong></p> </td> <td style="text-align: center;"> <p>< 0.1%</p> </td> <td style="text-align: center;"> <p>0.2%</p> </td> <td style="text-align: center;"> <p>1%</p> </td> <td style="text-align: center;"> <p>0.5%</p> </td> </tr> <tr valign="middle" style="background-color: #fff;"> <td> <p><strong>Nuclei isolation</strong></p> </td> <td style="text-align: center;"> <p>Yes</p> </td> <td style="text-align: center;"> <p>Yes</p> </td> <td style="text-align: center;"> <p>No</p> </td> <td style="text-align: center;"> <p>Yes</p> </td> </tr> <tr valign="middle" style="background-color: #fff;"> <td> <p><strong>Allows for shearing of... cells/tissue</strong></p> </td> <td style="text-align: center;"> <p>100 million cells</p> </td> <td style="text-align: center;"> <p>100 million cells</p> </td> <td style="text-align: center;"> <p>100 million cells</p> </td> <td style="text-align: center;"> <p>up to 25 g of tissue</p> </td> </tr> <tr valign="middle" style="background-color: #fff;"> <td> <p><strong>Corresponding to shearing buffers from</strong></p> </td> <td style="text-align: center;"> <p><a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p> </td> <td style="text-align: center;"> <p><a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p> <p><a href="https://www.diagenode.com/en/p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p> </td> <td style="text-align: center;"> <p><a href="https://www.diagenode.com/en/p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p> </td> <td style="text-align: center;"> <p><a href="https://www.diagenode.com/en/p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant ChIP-seq kit</a></p> </td> </tr> </tbody> </table> <p><em><span style="font-weight: 400;">Table comes from our </span><a href="https://www.diagenode.com/protocols/bioruptor-pico-chromatin-preparation-guide"><span style="font-weight: 400;">Guide for successful chromatin preparation using the Bioruptor® Pico</span></a></em></p> </div> </div> </div>', 'in_footer' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'slug' => 'chromatin-shearing', 'meta_keywords' => 'Chromatin shearing,Chromatin Immunoprecipitation,Bioruptor,Sonication,Sonicator', 'meta_description' => 'Diagenode's Bioruptor® is the instrument of choice for chromatin shearing used for a number of downstream applications such as qPCR and ChIP-seq that require optimally sheared, unbiased chromatin.', 'meta_title' => 'Chromatin shearing using Bioruptor® sonication device | Diagenode', 'modified' => '2017-11-15 10:14:02', 'created' => '2015-03-05 15:56:30', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '67', 'position' => '1', 'parent_id' => '6', 'name' => 'Bioruptor<sup>®</sup> Plus (and Standard) accessories', 'description' => '<p><span>All you need for the smooth running of the Bioruptor</span><sup><span>®</span></sup><span> Plus (or Standard). 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Researchers often overlook the critical nature of both of these steps. Eliminating inconsistencies in the shearing step, <strong>Diagenode's Bioruptor</strong><sup>®</sup> uses state-of-the-art ultrasound <strong>ACT</strong> (<strong>A</strong>daptive <strong>C</strong>avitation <strong>T</strong>echnology) to efficiently shear chromatin. ACT enables the highest chromatin quality for high IP efficiency and sensitivity for ChIP experiments with gentle yet highly effective shearing forces. Additionally, the Bioruptor<sup>®</sup> provides a precisely controlled temperature environment that preserves chromatin from heat degradation such that protein-DNA complexes are well-preserved for sensitive, unbiased, and accurate ChIP.<br /><br /> <strong>Diagenode's Bioruptor</strong><sup>®</sup> is the instrument of choice for chromatin shearing used for a number of downstream applications such as qPCR and ChIP-seq that require optimally sheared, unbiased chromatin.</div> <div class="small-12 medium-12 large-12 columns text-center"><br /><img src="https://www.diagenode.com/img/applications/pico_dna_shearing_fig2.png" /></div> <div class="small-10 medium-10 large-10 columns end small-offset-1"><small> <br /><strong>Panel A, 10 µl volume:</strong> Chromatin samples are sheared for 10, 20 and 30 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using 0.1 ml Bioruptor® Microtubes (Cat. No. B01200041). <strong>Panel B, 100 µl volume:</strong> Chromatin samples are sheared for 10 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using 0.65 ml Bioruptor® Microtubes (Cat. No. WA-005-0500). <strong>Panel C, 300 µl volume:</strong> Chromatin samples are sheared for 5, 10 and 15 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using using 1.5 ml Bioruptor microtubes (Cat. No. C30010016). Prior to de-crosslinking, samples are treated with RNase cocktail mixture at 37°C during 1 hour. The sheared chromatin is then de-crosslinked overnight and phenol/chloroform purified as described in the kit manual. 10 µl of DNA (equivalent of 500, 000 cells) are analyzed on a 2% agarose gel (MW corresponds to the 100 bp DNA molecular weight marker).</small></div> <div class="small-12 medium-12 large-12 columns"><br /><br /></div> <div class="small-12 medium-12 large-12 columns"> <p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p> </div> <div class="small-12 medium-12 large-12 columns"> <div class="page" title="Page 7"> <table> <tbody> <tr valign="middle"> <td></td> <td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histone)</a></strong></td> <td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td> <td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td> <td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-medium-sds-100-million-cells">Chromatin Shearing Kit (for Plant)</a></strong></td> </tr> <tr valign="middle" style="background-color: #fff;"> <td> <p><strong>SDS concentration</strong></p> </td> <td style="text-align: center;"> <p>< 0.1%</p> </td> <td style="text-align: center;"> <p>0.2%</p> </td> <td style="text-align: center;"> <p>1%</p> </td> <td style="text-align: center;"> <p>0.5%</p> </td> </tr> <tr valign="middle" style="background-color: #fff;"> <td> <p><strong>Nuclei isolation</strong></p> </td> <td style="text-align: center;"> <p>Yes</p> </td> <td style="text-align: center;"> <p>Yes</p> </td> <td style="text-align: center;"> <p>No</p> </td> <td style="text-align: center;"> <p>Yes</p> </td> </tr> <tr valign="middle" style="background-color: #fff;"> <td> <p><strong>Allows for shearing of... cells/tissue</strong></p> </td> <td style="text-align: center;"> <p>100 million cells</p> </td> <td style="text-align: center;"> <p>100 million cells</p> </td> <td style="text-align: center;"> <p>100 million cells</p> </td> <td style="text-align: center;"> <p>up to 25 g of tissue</p> </td> </tr> <tr valign="middle" style="background-color: #fff;"> <td> <p><strong>Corresponding to shearing buffers from</strong></p> </td> <td style="text-align: center;"> <p><a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p> </td> <td style="text-align: center;"> <p><a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p> <p><a href="https://www.diagenode.com/en/p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p> </td> <td style="text-align: center;"> <p><a href="https://www.diagenode.com/en/p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p> </td> <td style="text-align: center;"> <p><a href="https://www.diagenode.com/en/p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant ChIP-seq kit</a></p> </td> </tr> </tbody> </table> <p><em><span style="font-weight: 400;">Table comes from our </span><a href="https://www.diagenode.com/protocols/bioruptor-pico-chromatin-preparation-guide"><span style="font-weight: 400;">Guide for successful chromatin preparation using the Bioruptor® Pico</span></a></em></p> </div> </div> </div>', 'in_footer' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'slug' => 'chromatin-shearing', 'meta_keywords' => 'Chromatin shearing,Chromatin Immunoprecipitation,Bioruptor,Sonication,Sonicator', 'meta_description' => 'Diagenode's Bioruptor® is the instrument of choice for chromatin shearing used for a number of downstream applications such as qPCR and ChIP-seq that require optimally sheared, unbiased chromatin.', 'meta_title' => 'Chromatin shearing using Bioruptor® sonication device | Diagenode', 'modified' => '2017-11-15 10:14:02', 'created' => '2015-03-05 15:56:30', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '67', 'position' => '1', 'parent_id' => '6', 'name' => 'Bioruptor<sup>®</sup> Plus (and Standard) accessories', 'description' => '<p><span>All you need for the smooth running of the Bioruptor</span><sup><span>®</span></sup><span> Plus (or Standard). 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Researchers often overlook the critical nature of both of these steps. Eliminating inconsistencies in the shearing step, <strong>Diagenode's Bioruptor</strong><sup>®</sup> uses state-of-the-art ultrasound <strong>ACT</strong> (<strong>A</strong>daptive <strong>C</strong>avitation <strong>T</strong>echnology) to efficiently shear chromatin. ACT enables the highest chromatin quality for high IP efficiency and sensitivity for ChIP experiments with gentle yet highly effective shearing forces. Additionally, the Bioruptor<sup>®</sup> provides a precisely controlled temperature environment that preserves chromatin from heat degradation such that protein-DNA complexes are well-preserved for sensitive, unbiased, and accurate ChIP.<br /><br /> <strong>Diagenode's Bioruptor</strong><sup>®</sup> is the instrument of choice for chromatin shearing used for a number of downstream applications such as qPCR and ChIP-seq that require optimally sheared, unbiased chromatin.</div> <div class="small-12 medium-12 large-12 columns text-center"><br /><img src="https://www.diagenode.com/img/applications/pico_dna_shearing_fig2.png" /></div> <div class="small-10 medium-10 large-10 columns end small-offset-1"><small> <br /><strong>Panel A, 10 µl volume:</strong> Chromatin samples are sheared for 10, 20 and 30 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using 0.1 ml Bioruptor® Microtubes (Cat. No. B01200041). <strong>Panel B, 100 µl volume:</strong> Chromatin samples are sheared for 10 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using 0.65 ml Bioruptor® Microtubes (Cat. No. WA-005-0500). <strong>Panel C, 300 µl volume:</strong> Chromatin samples are sheared for 5, 10 and 15 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using using 1.5 ml Bioruptor microtubes (Cat. No. C30010016). Prior to de-crosslinking, samples are treated with RNase cocktail mixture at 37°C during 1 hour. The sheared chromatin is then de-crosslinked overnight and phenol/chloroform purified as described in the kit manual. 10 µl of DNA (equivalent of 500, 000 cells) are analyzed on a 2% agarose gel (MW corresponds to the 100 bp DNA molecular weight marker).</small></div> <div class="small-12 medium-12 large-12 columns"><br /><br /></div> <div class="small-12 medium-12 large-12 columns"> <p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p> </div> <div class="small-12 medium-12 large-12 columns"> <div class="page" title="Page 7"> <table> <tbody> <tr valign="middle"> <td></td> <td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histone)</a></strong></td> <td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td> <td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td> <td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-medium-sds-100-million-cells">Chromatin Shearing Kit (for Plant)</a></strong></td> </tr> <tr valign="middle" style="background-color: #fff;"> <td> <p><strong>SDS concentration</strong></p> </td> <td style="text-align: center;"> <p>< 0.1%</p> </td> <td style="text-align: center;"> <p>0.2%</p> </td> <td style="text-align: center;"> <p>1%</p> </td> <td style="text-align: center;"> <p>0.5%</p> </td> </tr> <tr valign="middle" style="background-color: #fff;"> <td> <p><strong>Nuclei isolation</strong></p> </td> <td style="text-align: center;"> <p>Yes</p> </td> <td style="text-align: center;"> <p>Yes</p> </td> <td style="text-align: center;"> <p>No</p> </td> <td style="text-align: center;"> <p>Yes</p> </td> </tr> <tr valign="middle" style="background-color: #fff;"> <td> <p><strong>Allows for shearing of... cells/tissue</strong></p> </td> <td style="text-align: center;"> <p>100 million cells</p> </td> <td style="text-align: center;"> <p>100 million cells</p> </td> <td style="text-align: center;"> <p>100 million cells</p> </td> <td style="text-align: center;"> <p>up to 25 g of tissue</p> </td> </tr> <tr valign="middle" style="background-color: #fff;"> <td> <p><strong>Corresponding to shearing buffers from</strong></p> </td> <td style="text-align: center;"> <p><a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p> </td> <td style="text-align: center;"> <p><a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p> <p><a href="https://www.diagenode.com/en/p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p> </td> <td style="text-align: center;"> <p><a href="https://www.diagenode.com/en/p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p> </td> <td style="text-align: center;"> <p><a href="https://www.diagenode.com/en/p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant ChIP-seq kit</a></p> </td> </tr> </tbody> </table> <p><em><span style="font-weight: 400;">Table comes from our </span><a href="https://www.diagenode.com/protocols/bioruptor-pico-chromatin-preparation-guide"><span style="font-weight: 400;">Guide for successful chromatin preparation using the Bioruptor® Pico</span></a></em></p> </div> </div> </div>', 'in_footer' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'slug' => 'chromatin-shearing', 'meta_keywords' => 'Chromatin shearing,Chromatin Immunoprecipitation,Bioruptor,Sonication,Sonicator', 'meta_description' => 'Diagenode's Bioruptor® is the instrument of choice for chromatin shearing used for a number of downstream applications such as qPCR and ChIP-seq that require optimally sheared, unbiased chromatin.', 'meta_title' => 'Chromatin shearing using Bioruptor® sonication device | Diagenode', 'modified' => '2017-11-15 10:14:02', 'created' => '2015-03-05 15:56:30', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '67', 'position' => '1', 'parent_id' => '6', 'name' => 'Bioruptor<sup>®</sup> Plus (and Standard) accessories', 'description' => '<p><span>All you need for the smooth running of the Bioruptor</span><sup><span>®</span></sup><span> Plus (or Standard). 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Researchers often overlook the critical nature of both of these steps. Eliminating inconsistencies in the shearing step, <strong>Diagenode's Bioruptor</strong><sup>®</sup> uses state-of-the-art ultrasound <strong>ACT</strong> (<strong>A</strong>daptive <strong>C</strong>avitation <strong>T</strong>echnology) to efficiently shear chromatin. ACT enables the highest chromatin quality for high IP efficiency and sensitivity for ChIP experiments with gentle yet highly effective shearing forces. Additionally, the Bioruptor<sup>®</sup> provides a precisely controlled temperature environment that preserves chromatin from heat degradation such that protein-DNA complexes are well-preserved for sensitive, unbiased, and accurate ChIP.<br /><br /> <strong>Diagenode's Bioruptor</strong><sup>®</sup> is the instrument of choice for chromatin shearing used for a number of downstream applications such as qPCR and ChIP-seq that require optimally sheared, unbiased chromatin.</div> <div class="small-12 medium-12 large-12 columns text-center"><br /><img src="https://www.diagenode.com/img/applications/pico_dna_shearing_fig2.png" /></div> <div class="small-10 medium-10 large-10 columns end small-offset-1"><small> <br /><strong>Panel A, 10 µl volume:</strong> Chromatin samples are sheared for 10, 20 and 30 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using 0.1 ml Bioruptor® Microtubes (Cat. No. B01200041). <strong>Panel B, 100 µl volume:</strong> Chromatin samples are sheared for 10 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using 0.65 ml Bioruptor® Microtubes (Cat. No. WA-005-0500). <strong>Panel C, 300 µl volume:</strong> Chromatin samples are sheared for 5, 10 and 15 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using using 1.5 ml Bioruptor microtubes (Cat. No. C30010016). Prior to de-crosslinking, samples are treated with RNase cocktail mixture at 37°C during 1 hour. The sheared chromatin is then de-crosslinked overnight and phenol/chloroform purified as described in the kit manual. 10 µl of DNA (equivalent of 500, 000 cells) are analyzed on a 2% agarose gel (MW corresponds to the 100 bp DNA molecular weight marker).</small></div> <div class="small-12 medium-12 large-12 columns"><br /><br /></div> <div class="small-12 medium-12 large-12 columns"> <p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p> </div> <div class="small-12 medium-12 large-12 columns"> <div class="page" title="Page 7"> <table> <tbody> <tr valign="middle"> <td></td> <td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histone)</a></strong></td> <td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td> <td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td> <td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-medium-sds-100-million-cells">Chromatin Shearing Kit (for Plant)</a></strong></td> </tr> <tr valign="middle" style="background-color: #fff;"> <td> <p><strong>SDS concentration</strong></p> </td> <td style="text-align: center;"> <p>< 0.1%</p> </td> <td style="text-align: center;"> <p>0.2%</p> </td> <td style="text-align: center;"> <p>1%</p> </td> <td style="text-align: center;"> <p>0.5%</p> </td> </tr> <tr valign="middle" style="background-color: #fff;"> <td> <p><strong>Nuclei isolation</strong></p> </td> <td style="text-align: center;"> <p>Yes</p> </td> <td style="text-align: center;"> <p>Yes</p> </td> <td style="text-align: center;"> <p>No</p> </td> <td style="text-align: center;"> <p>Yes</p> </td> </tr> <tr valign="middle" style="background-color: #fff;"> <td> <p><strong>Allows for shearing of... cells/tissue</strong></p> </td> <td style="text-align: center;"> <p>100 million cells</p> </td> <td style="text-align: center;"> <p>100 million cells</p> </td> <td style="text-align: center;"> <p>100 million cells</p> </td> <td style="text-align: center;"> <p>up to 25 g of tissue</p> </td> </tr> <tr valign="middle" style="background-color: #fff;"> <td> <p><strong>Corresponding to shearing buffers from</strong></p> </td> <td style="text-align: center;"> <p><a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p> </td> <td style="text-align: center;"> <p><a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p> <p><a href="https://www.diagenode.com/en/p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p> </td> <td style="text-align: center;"> <p><a href="https://www.diagenode.com/en/p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p> </td> <td style="text-align: center;"> <p><a href="https://www.diagenode.com/en/p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant ChIP-seq kit</a></p> </td> </tr> </tbody> </table> <p><em><span style="font-weight: 400;">Table comes from our </span><a href="https://www.diagenode.com/protocols/bioruptor-pico-chromatin-preparation-guide"><span style="font-weight: 400;">Guide for successful chromatin preparation using the Bioruptor® Pico</span></a></em></p> </div> </div> </div>', 'in_footer' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'slug' => 'chromatin-shearing', 'meta_keywords' => 'Chromatin shearing,Chromatin Immunoprecipitation,Bioruptor,Sonication,Sonicator', 'meta_description' => 'Diagenode's Bioruptor® is the instrument of choice for chromatin shearing used for a number of downstream applications such as qPCR and ChIP-seq that require optimally sheared, unbiased chromatin.', 'meta_title' => 'Chromatin shearing using Bioruptor® sonication device | Diagenode', 'modified' => '2017-11-15 10:14:02', 'created' => '2015-03-05 15:56:30', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '67', 'position' => '1', 'parent_id' => '6', 'name' => 'Bioruptor<sup>®</sup> Plus (and Standard) accessories', 'description' => '<p><span>All you need for the smooth running of the Bioruptor</span><sup><span>®</span></sup><span> Plus (or Standard). 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