Diagenode

GATA6 is predicted to regulate DNA methylation in an in vitro model ofhuman hepatocyte differentiation.


Suzuki T. et al.

Hepatocytes are the dominant cell type in the human liver, with functions in metabolism, detoxification, and producing secreted proteins. Although gene regulation and master transcription factors involved in the hepatocyte differentiation have been extensively investigated, little is known about how the epigenome is regulated, particularly the dynamics of DNA methylation and the critical upstream factors. Here, by examining changes in the transcriptome and the methylome using an in vitro hepatocyte differentiation model, we show putative DNA methylation-regulating transcription factors, which are likely involved in DNA demethylation and maintenance of hypo-methylation in a differentiation stage-specific manner. Of these factors, we further reveal that GATA6 induces DNA demethylation together with chromatin activation in a binding-site-specific manner during endoderm differentiation. These results provide an insight into the spatiotemporal regulatory mechanisms exerted on the DNA methylation landscape by transcription factors and uncover an epigenetic role for transcription factors in early liver development.

Tags
IP-Star Compact
iDeal ChIP-seq Kit for Transcription Factors
ChIPmentation

Share this article

Published
May, 2022

Source

Products used in this publication

  • ChIP kit icon
    C01010055
    iDeal ChIP-seq kit for Transcription Factors
  • ChIP kit icon
    C01010058
    Auto iDeal ChIP-seq Kit for Transcription Factors
  • ChIP kit icon
    C01010172
    Auto iDeal ChIP-seq Kit for Transcription Factors
  • default alt
    C01011030
    TAG Kit for ChIPmentation

Events

 See all events

News

 See all news


The European Regional Development Fund and Wallonia are investing in your future.

Extension of industrial buildings and new laboratories.


       Site map   |   Contact us   |   Conditions of sales   |   Conditions of purchase   |   Privacy policy   |   Diagenode Diagnostics