Diagenode

CRISPR/Cas9 editing: mutation detection with mismatch cleavage assay

Genome editing using CRISPR/Cas9 is used for targeted mutagenesis. But because genome editing does not target all loci with similar efficiencies, the mutation hit-rate at a given locus needs to be evaluated. The analysis of mutants obtained using CRISPR/Cas9 requires specific methods for mutation detection, and the enzyme mismatch cleavage method is used commonly for this purpose.

Related product

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    C29010001
    Cas9 Nuclease protein NLS

Events

  • The 12th Annual Meeting of the Japanese Society for Epigenetics
    Jun 24-Jun 25, 2018
  • PEMED 2018 Personalized and Precision Medecine
    Asiem Paris France
    Jun 25-Jun 27, 2018
  • FAANG Training school on ChIP-seq
    Wageningen
    Jun 25-Jun 29, 2018
  • Ecole Thematique FieldEpiGen
    Perpignan
    Jun 25-Jun 29, 2018
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Applications

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