CRISPR/Cas9 editing: mutation detection with mismatch cleavage assay

Genome editing using CRISPR/Cas9 is used for targeted mutagenesis. But because genome editing does not target all loci with similar efficiencies, the mutation hit-rate at a given locus needs to be evaluated. The analysis of mutants obtained using CRISPR/Cas9 requires specific methods for mutation detection, and the enzyme mismatch cleavage method is used commonly for this purpose.