Diagenode

WDR5 polyclonal antibody - Classic

Histone-Deacetylase-polyclonal-antibody-diagenode
Catalog Number
Format
Price
C15410027
(pAb-027-050)
50 µg/25 µl
$295.00
  Bulk order

Alternative names: BIG3, SWD3

Polyclonal antibody raised in rabbit against human WDR5 (WD (tryptophan-aspartate) repeat domain 5), using a recombinant protein.

Lot001
Concentration2.0 µg/µl
Species reactivityHuman
TypePolyclonal
PurityProtein G purified
HostRabbit
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ChIP* 2 μg per ChIP Fig 1, 2
Western Blotting 1:1,000 Fig 3
Immunofluorescence 1:1,000 Fig 4

*Please note that the optimal antibody amount per ChIP should be determined by the end-user. We recommend testing 1-5 μg per ChIP.

  • Validation Data

    ChIP

    Figure 1. ChIP results obtained with the Diagenode antibody directed against WDR5
    ChIP assays were performed using HeLa cells, the Diagenode antibody against WDR5 (Cat. No. C15410027) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2, 5 and 10 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with optimized primers for the promoters of the RPL41 and RPS4X ribosomal protein genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

    ChIP-seq figure A

    ChIP-seq figure B

    ChIP-seq figure C

    ChIP-seq figure D

    Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against WDR5
    ChIP was performed on sheared chromatin from 4 million HeLa cells using 2 μg of the Diagenode antibody against WDR5 (Cat. No. C15410027) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 2 Mb region of human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the RPL41 and RPS4X positive control genes (fig 2C and D).

    Western blot

    Figure 3. Western blot analysis using the Diagenode antibody directed against WDR5
    Nclear extracts from HeLa cells (20 μg) were analysed by Western blot using the Diagenode antibody against WDR5 (Cat. No. C15410027) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.

    IF

    Figure 4. Immunofluorescence using the Diagenode antibody directed against WDR
    HeLa cells were stained with the Diagenode antibody against WDR5 (Cat. No. C15410027) and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the WDR5 antibody (left) diluted 1:1,000 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.

  • Applications
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    IF
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
    ChIP-seq (ab)
    Read more
    ChIP-qPCR (ab)
    Read more
  • Documents
    Datasheet WDR5 pAb-027-050 DATASHEET
    Datasheet description
    Download
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
    Download
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
    Download
  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: WDR5 polyclonal antibody - Classic (Diagenode Cat# C15410027 Lot# 001). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

  • Related products

Events

  • "Molecular Biosystems" Conference on Eukaryotic Gene Regulation and Functional Genomics
    Puerto Varas, Región de Los Lagos, Chile
    Sep 23-Sep 26, 2017
 See all events

Twitter feed

News

 See all news