WDR5 polyclonal antibody - Pioneer

Catalog Number
50 µg/25 µl
  Bulk order

Alternative names: BIG3, SWD3

Polyclonal antibody raised in rabbit against human WDR5 (WD (tryptophan-aspartate) repeat domain 5), using a recombinant protein.

Concentration2.0 µg/µl
Species reactivityHuman
PurityProtein G purified
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
Western Blotting 1:1,000 Fig 1
Immunofluorescence 1:1,000 Fig 2
  • Validation Data

    Western blot

    Figure 1. Western blot analysis using the Diagenode antibody directed against WDR5
    HeLa nuclear extract (HeLa NE, [20 μg]) was analysed by Western blot using the Diagenode antibody against WDR5 (Cat. No. pAb-027-050) diluted of 1:1000 in TBS-Tween containing 5% skimmed milk (lane 2). The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest is indicated on the right.


    Figure 2. Immunofluorescence using the Diagenode antibody directed against WDR
    HeLa cells were stained with the Diagenode antibody against WDR5 (Cat. No. C15410027) and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the WDR5 antibody (left) diluted 1:1,000 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.

  • Applications
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
  • Documents
    Datasheet WDR5 pAb-027-050 DATASHEET
    Datasheet description
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: WDR5 polyclonal antibody - Pioneer (Diagenode Cat# C15410027 Lot# 001). Click here to copy to clipboard.

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