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Figure 1. Western blot analysis using the Diagenode antibody directed against SKI8 Western blot was performed on nuclear extract from HeLa cells (lane 1), Drosophila adult whole cell extracts (lane 2), and the purified human PAF complex (lane 3) (Figure 1A) or on nuclear extracts from HeLa cells (HeLa NE, 20 μg) (Figure 1B), using the Diagenode antibody against SKI8 (Cat. No. pAb-012-150) diluted 1:1,000. The location of the protein is indicated on the right, the marker (in kDa) is shown on the left.
WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.
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Regulation of transcriptional elongation in pluripotency and cell differentiation by the PHD-finger protein Phf5a Strikoudis A. et al. Pluripotent embryonic stem cells (ESCs) self-renew or differentiate into all tissues of the developing embryo and cell-specification factors are necessary to balance gene expression. Here we delineate the function of the PHD-finger protein 5a (Phf5a) in ESC self-renewal and ascribe its role in regulating pluripotenc...
