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RFX-AP polyclonal antibody - Classic

Catalog Number
50 µg/50 µl
  Bulk order

Polyclonal antibody raised in rabbit against RFXAP (Regulatory factor X-associated protein), using the recombinant protein.

Concentration1.0 µg/µl
Species reactivityHuman
PurityAffinity purified
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ChIP * 5-7 μg/IP Fig 1

* Please note that of the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-10 μg per IP.

  • Validation Data

    ChIP results

    Figure 1 ChIP results obtained with the Diagenode antibody directed against RFXAP
    ChIP assays were performed using NALM cells (a cell line derived from human pre-B leukemia), the Diagenode antibody against RFXAP (cat. No. pAb-061-050) and optimized primer sets for PCR. Sheared chromatin from 2 million cells and respectively 5 and 7 μg of antibody were used per ChIP experiment. An anti-flag antibody (lane 2) was used as negative IP control. Figure 1 shows the result of the end-point PCR with primers for HLA_DRA, used as positive control (lower panel) and for HLA_DOB, used a negative PCR control (upper panel).

  •  Applications
  •  Documents
    Datasheet RFXAP pAb-061-050 DATASHEET
    Datasheet description
  •  Publications

    How to properly cite this product in your work

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