RFX-AP Antibody

Catalog Number
50 µg/50 µl
  Bulk order

Polyclonal antibody raised in rabbit against RFXAP (Regulatory factor X-associated protein), using the recombinant protein.

Concentration1.0 µg/µl
Species reactivityHuman
PurityAffinity purified
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ChIP * 5-7 μg/IP Fig 1

* Please note that of the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-10 μg per IP.

  • Validation Data

    RFX-AP Antibody ChIP Grade

    Figure 1 ChIP results obtained with the Diagenode antibody directed against RFXAP
    ChIP assays were performed using NALM cells (a cell line derived from human pre-B leukemia), the Diagenode antibody against RFXAP (cat. No. pAb-061-050) and optimized primer sets for PCR. Sheared chromatin from 2 million cells and respectively 5 and 7 μg of antibody were used per ChIP experiment. An anti-flag antibody (lane 2) was used as negative IP control. Figure 1 shows the result of the end-point PCR with primers for HLA_DRA, used as positive control (lower panel) and for HLA_DOB, used a negative PCR control (upper panel).

  • Target Description

    RFXAP (UniProtKB/Swiss-Prot entry O00287) is part of the RFX complex that binds to the X-box of MHC II promoters. The RFX complex consists of at least 3 different subunits; RFXAP, RFX5 and RFX-B/RFXANK; with each subunit representing a separate complementation group. RFX forms cooperative DNA binding complexes with X2BP and CBF/NF-Y. RFX associates with CIITA to form an active transcriptional complex. Defects in RFXAP can cause bare lymphocyte syndrome type II (BLS II); also known as hereditary MHC class II deficiency or HLA class II-deficient combined immunodeficiency.

  •  Applications
  •  Documents
    Datasheet RFXAP pAb-061-050 DATASHEET
    Datasheet description
  •  Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: RFX-AP Antibody (Diagenode Cat# C15410061 Lot# 001). Click here to copy to clipboard.

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