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PHF8 Antibody - ChIP Grade

Catalog Number
100 μl
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Polyclonal antibody raised in rabbit against human PHF8 (PHD finger protein 8), using a synthetic peptide containing a sequence from the central part of the protein.

Concentration0.2 μg/μl
Species reactivityHuman
TypePolyclonal ChIP grade
PurityAffinity purified
Storage ConditionsStore at 4°C
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ChIP * 1-2 μg/ChIP Fig 1
Western Blotting 1:1,000 Fig 2
IP 6 μg per IP Fig 3

* Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μg per IP.

1Manufactured by Bethyl Laboratories, Inc., Texas, USA

  • Validation data

    PHF8 Antibody ChIP Grade

    Figure 1. ChIP results obtained with the Diagenode antibody directed against PHF8
    ChIP assays were performed using HeLa cells, the Diagenode antibody against PHF8 (cat. No. C15410336) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1 and 2 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the EIF2S3 and EIF4A2 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

    PHF8 Antibody validated in Western Blot

    Figure 2. Western blot analysis using the Diagenode antibody directed against PHF8
    Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against PHF8 (Cat. No. C15410336) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.

    PHF8 Antibody validated in Immunoprecipitation

    Figure 3. Immunoprecipitation analysis using the Diagenode antibody directed against PHF8
    Immunoprecipitation was performed on whole cell extracts from HeLa cells using 6 μg of the Diagenode antibody against PHF8 (Cat. No. C15410336, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated PHF8 protein was detected by western blot with the PHF8 antibody diluted 1:500.

  • Target Description

    PHF8 (UniProtKB/Swiss-Prot entry Q9UPP1) is a jumonji domain containing protein which plays role in histone demethylation. PHF8 selectively demethylates the di- and monomethyl H3K9, H3K27 and H4K20 and acts as a transcriptional activator. It plays a key role cell cycle progression, rDNA transcription and brain development. Mutations in PHF8 lead to Siderius type X-linked mental retardation (MRXSSD), a mild to borderline type of mental retardation.

  •  Applications
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    Immunoprecipitation Read more
    ChIP-qPCR (ab)
    Read more
  •  Documents
    PHF8 polyclonal antibody DATASHEET
    Polyclonal antibody raised in rabbit against human PHF8 (PHD finger protein 8), using a synthetic...
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
  •  Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: PHF8 Antibody - ChIP Grade (Diagenode Cat# C15410336 Lot# A301-772A3). Click here to copy to clipboard.

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