Diagenode

PHF8 polyclonal antibody

Antibody ChIP icon
Catalog Number
Format
Price
C15410336
100 μl
$305.00
  Bulk order

Polyclonal antibody raised in rabbit against human PHF8 (PHD finger protein 8), using a synthetic peptide containing a sequence from the central part of the protein.

1Manufactured by Bethyl Laboratories, Inc., Texas, USA

LotA301-772A3
Concentration0.2 μg/μl
Species reactivityHuman
TypePolyclonal ChIP grade
PurityAffinity purified
HostRabbit
Storage ConditionsStore at 4°C
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ChIP * 1-2 μg/ChIP Fig 1
Western Blotting 1:1,000 Fig 2
IP 6 μg per IP Fig 3

* Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μg per IP.

  • Validation data

    ChIP

    Figure 1. ChIP results obtained with the Diagenode antibody directed against PHF8
    ChIP assays were performed using HeLa cells, the Diagenode antibody against PHF8 (cat. No. C15410336) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1 and 2 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the EIF2S3 and EIF4A2 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

    WB

    Figure 2. Western blot analysis using the Diagenode antibody directed against PHF8
    Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against PHF8 (Cat. No. C15410336) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.

    IP

    Figure 3. Immunoprecipitation analysis using the Diagenode antibody directed against PHF8
    Immunoprecipitation was performed on whole cell extracts from HeLa cells using 6 μg of the Diagenode antibody against PHF8 (Cat. No. C15410336, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated PHF8 protein was detected by western blot with the PHF8 antibody diluted 1:500.

  • Applications
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    IP
    Immunoprecipitation Read more
    ChIP-qPCR (ab)
    Read more
  • Documents
    PHF8 polyclonal antibody DATASHEET
    Polyclonal antibody raised in rabbit against human PHF8 (PHD finger protein 8), using a synthetic...
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    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
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    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
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  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: PHF8 polyclonal antibody (Diagenode Cat# C15410336 Lot# A301-772A3). Click here to copy to clipboard.

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Events

  • The 12th Annual Meeting of the Japanese Society for Epigenetics
    Jun 24-Jun 25, 2018
  • PEMED 2018 Personalized and Precision Medecine
    Asiem Paris France
    Jun 25-Jun 27, 2018
  • FAANG Training school on ChIP-seq
    Wageningen
    Jun 25-Jun 29, 2018
  • Ecole Thematique FieldEpiGen
    Perpignan
    Jun 25-Jun 29, 2018
 See all events

Applications

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