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LwaCas13a Antibody

Catalog Number
Format
Price
C15100149
100 µl
$440.00
  Bulk order
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Monoclonal antibody raised in mouse against the Cas13a protein from Leptotrichia wadei. (CRISPR associated protein 13, Jennifer Doudna form) using a recombinant protein containing the N-terminus of the protein.

Lot001
ConcentrationNot determined
Species reactivityLeptotrichia wadei
TypeMonoclonal
PurityConcentrated cell culture supernatant.
HostMouse
Storage ConditionsStore at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.
Storage BufferTBS containing 0.02 % Na-azide.
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
Western blotting 1:500 Fig 1
  • Validation data
    LwaCas13a Antibody validated for Western Blot

    Western blot analysis using the Diagenode monoclonal antibody directed against LwaCas13a
    Western blot was performed on 20 µg protein extracts from HEK293 cells (lane 1), HEK293 cells spiked with 1 ng recombinant LwaCas13a protein (lane 2) and HEK293 cells transiently expressing full-length Cas13b (lane 3), using the Diagenode antibody against LwaCas13a (Cat. No. C15100149). The antibody was diluted 1:500 in PBS-T containing 0.5% NFDM. The marker is shown on the left, the position of the Cas13b protein is indicated on the right. The bottom panel shows the result of a WB performed with an actin antibody, used as a loading control.

  • Target Description

    CRISPR systems are adaptable immune mechanisms which are present in many bacteria to protect themselves from foreign nucleic acids, such as viruses, transposable elements or plasmids. The CRISPR/Cas9 (CRISPRassociated protein 9 nuclease) system from S. pyogenes was the first to be adapted for inducing sequencespecific double stranded breaks and targeted genome editing. This system is unique and flexible due to its dependence on RNA as the moiety that targets the nuclease to a desired DNA sequence and can be used to induce indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation. Recently, a socalled class 2 type VI CRISPR system has been identified which is characterized by the presence of the single effector protein. Cas13a is one of the proteins belonging to the class 2 type VI system identified so far. These proteins lack homology to other DNA nuclease domains but contain two Higher Eukaryotes and Prokaryotes Nucleotide-binding (HEPN) domains and are thought to function solely as an RNA-guided RNA-targeting CRISPR effector.

  •  Applications
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
  •  Documents
    LwaCas13a monoclonal antibody datasheet DATASHEET
    Monoclonal antibody raised in mouse against the Cas13a protein from Leptotrichia wadei. (CRISPR a...
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  •  Publications

    How to properly cite this product in your work

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