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Monoclonal antibody raised in mouse against the Cas13a protein from Leptotrichia wadei. (CRISPR associated protein 13, Jennifer Doudna form) using a recombinant protein containing the N-terminus of the protein.
Concentrated cell culture supernatant.
Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.
TBS containing 0.02 % Na-azide.
This product is for research use only. Not for use in diagnostic or therapeutic procedures.
Western blot analysis using the Diagenode monoclonal antibody directed against LwaCas13a Western blot was performed on 20 µg protein extracts from HEK293 cells (lane 1), HEK293 cells spiked with 1 ng recombinant LwaCas13a protein (lane 2) and HEK293 cells transiently expressing full-length Cas13b (lane 3), using the Diagenode antibody against LwaCas13a (Cat. No. C15100149). The antibody was diluted 1:500 in PBS-T containing 0.5% NFDM. The marker is shown on the left, the position of the Cas13b protein is indicated on the right. The bottom panel shows the result of a WB performed with an actin antibody, used as a loading control.
WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.
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