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Figure 1. Western blot analysis using the Diagenode antibody directed against hLeo1 To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human JMJD2c (Cat. No. CS-105-100). The wells were coated with the peptide used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:2,000.
WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.
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