HDAC3 Antibody

Catalog Number
50 μg/25 μl
  Bulk order

Alternative names: HD3, RPD3, RPD3-2, SMAP45

Monoclonal antibody raised in mouse against human HDAC3 (Histone deacetylase 3), using a KLH-conjugated synthetic peptide containing a sequence from the C-terminal region of the protein.

Concentration2.0 µg/µl
Species reactivityHuman
PurityProtein A purified monoclonal antibody
Storage ConditionsStore at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.
Storage BufferPBS containing 0.05% azide and 0.05% ProClin 300.
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
Immunofluorescence 1:500 Fig 1
  • Validation Data

    HDAC3 Antibody validated in Immunofluorescence

    Figure 1. Immunofluorescence using the Diagenode monoclonal antibody directed against HDAC3
    HeLa cells were stained with the Diagenode antibody against HDAC3 (Cat. No. C15200145) and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the HDAC3 antibody (left) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa594. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.

  • Target Description

    HDAC3 (UniProt/Swiss-Prot entry O15379) catalyses the deacetylation of lysine residues in the N-terminal part of the core histones (H2A, H2B, H3 and H4). Acetylation and deacetylation of these highly conserved lysine residues is important for the control of gene expression and HDAC activity is associated with gene repression. Histone deacetylation is established by the formation of large multiprotein complexes. HDAC3 may bind to the zinc-finger transcription factor YY1, thereby regulating transcription. It is also able to modulate cell growth and apoptosis through the interaction with p53 and is thought to be essential for the repression of the POU1F1 transcription factor.

  •  Applications
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
    ChIP-qPCR (ab)
    Read more
  •  Documents
    Datasheet HDAC3 MAb-145-050 DATASHEET
    Datasheet description
  •  Safety sheets
    HDAC3 Antibody SDS GB en Download
    HDAC3 Antibody SDS US en Download
    HDAC3 Antibody SDS BE nl Download
    HDAC3 Antibody SDS BE fr Download
    HDAC3 Antibody SDS FR fr Download
    HDAC3 Antibody SDS ES es Download
    HDAC3 Antibody SDS DE de Download
    HDAC3 Antibody SDS JP ja Download
  •  Publications

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