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H4K16ac Antibody - ChIP Grade (sample size)

Catalog Number
Format
Price
C15200219-10
10 μg
$95.00
  Bulk order
Other format



Monoclonal antibody raised in mouse against histone H4, acetylated at lysine 16 (H4K16ac), using a KLH-conjugated synthetic peptide.

Lot001-11
Concentration1 μg/μl
Species reactivityhuman
TypeMonoclonal
PurityProtein A purified
HostMouse
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ChIP * 1-2 μg/ChIP Figure 1
Dot Blotting 1:10,000 Figure 2
Western Blotting 1:500 Figure 2

* Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μg per IP.

  • Validation Data

    H4K12ac Antibody ChIP Grade

    Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against H4K16ac
    ChIP assays were performed using HeLa cells, the monoclonal antibody against H4K16ac (Cat. No. C15200219) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 1.5 million cells. A titration of the antibody consisting of 0.5, 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the coding region of the EIF2S3 gene, used as a positive control, and for the Sat2 satellite repeat, used as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis)

    H4K12ac Antibody validated in Dot Blot

    Figure 2. Cross reactivity tests using the Diagenode monoclonal antibody directed against H4K16ac
    To test the cross reactivity of the Diagenode monoclonal antibody against H4K16ac (Cat. No. C15200219), a Dot Blot analysis was performed with peptides containing other acetylations and unmodified sequences of histone H4. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:10,000. Figure 2 shows a high specificity of the antibody for the modification of interest.

    H4K12ac Antibody validated in Western Blot

    Figure 3. Western blot analysis using the Diagenode monoclonal antibody directed against H4K16ac
    Histone extracts (15 μg) from HeLa cells were analysed by Western blot using the Diagenode monoclonal antibody against H4K16ac (Cat. No. C15200219) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.

  • Target Description

    Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the so-called “histone code”. Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Acetylation of histone H4 is associated with active genes.

  •  Applications
    DB
    Dot blotting Read more
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    ChIP-qPCR (ab)
    Read more
  •  Documents
    C15200219 H4K16ac monoclonal DATASHEET
    Datasheet description
    Download
  •  Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: H4K16ac Antibody - ChIP Grade (sample size) (Diagenode Cat# C15200219-10 Lot# 001-11). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    SIRT1-dependent epigenetic regulation of H3 and H4 histone acetylation in human breast cancer
    Khaldoun Rifaï et al.
    Breast cancer is the most frequently diagnosed malignancy in women worldwide. It is well established that the complexity of carcinogenesis involves profound epigenetic deregulations that contribute to the tumorigenesis process. Deregulated H3 and H4 acetylated histone marks are amongst those alterations. Sirtuin-1 (...

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