FLAG Antibody

Catalog Number
100 µg
  Bulk order

Monoclonal antibody raised in mouse against Flag™ (peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys)), using a KLH-conjugated synthetic peptide.

Concentration1 μg/μl
Species reactivityCarboxy and amino terminal linked FLAG™ tagged recombinant proteins
PurityProtein A purified
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ELISA 1:20,000 - 1:100,000
Western Blotting 1:2,000 - 1:20,000 Fig 1, 2
  • Validation Data

    Western Blot

    Figure 1. FLAG antibody western blot results
    Twenty-four (24) clones were randomly selected and grown up from glycerol stocks by inoculating 0.5mL 2xYT medium. Expression of recombinant proteins was induced by the addition of IPTG. Proteins were purified by nickel affinity chromatography and eluted in 40 μL. Samples were diluted 10-fold, transferred to nitrocellulose membrane and WB was performed with the FLAG monoclonal antibody.

    Western Blot

    Figure 2. FLAG antibody western blot results
    Monoclonal Antibody to detect FLAGTM conjugated proteins detects both C terminal linked and N terminal linked FLAGTM tagged recombinant proteins by western blot.

  •  Applications
    Enzyme-linked immunosorbent assay. Read more
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
  •  Documents
    Datasheet FLAG C15200209 DATASHEET
    Datasheet description
  •  Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: FLAG Antibody (Diagenode Cat# C15200209 Lot# 002). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    ThPOK is a critical multifaceted regulator of myeloid lineagedevelopment.
    Basu J. et al.
    The transcription factor ThPOK (encoded by Zbtb7b) is well known for its role as a master regulator of CD4 lineage commitment in the thymus. Here, we report an unexpected and critical role of ThPOK as a multifaceted regulator of myeloid lineage commitment, differentiation and maturation. Using reporter and knockout ...

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