This IP grade product is optimized to be used as carrier for DNA purification after chromatin immunoprecipitation (ChIP) and after methylated DNA immunoprecipitation (Methyl DNA IP). It is included in our Transcription ChIP KIT (cat# kch-redTBP-012), in our Histone ChIP KIT (Cat. No. kch-orgHIS-012) and in our Methylated DNA Immunoprecipitation KIT-MeDIP kit (Cat. No. mc-green-003). It is now separately available. Following IP and washes, the immunoprecipitated (IP’d) material is submitted to Phenol/Chloroform/Isomanyl alcohol (PCI) extraction. Then, to the aqueous phase obtained after PCI extraction of the IP’d DNA material, our DNA co-precipitant is added with IP grade DNA precipitant (Cat. No. kch-217-001) and ice-cold 96% ethanol.
ChIP-qPCR
Chromatin Immunoprecipitation (ChIP) coupled with quantitative PCR can be used to investigate protein-DNA interaction at known genomic binding sites. if sites are not known, qPCR primers can also be designed against potential regulatory region... Read more
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