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Figure 1. ChIP results obtained with the Diagenode antibody directed against CHD7 ChIP assays were performed using K562 cells, the Diagenode antibody against CHD7 (Cat. No. C15410340) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the active RUNX1 and GAPDH genes, used as positive control, and for the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against CHD7 ChIP was performed on sheared chromatin from 4 million K562 cells using 1 μg of the Diagenode antibody against CHD7 (Cat. No. C15410340) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 600 Kb region of the human X-chromosome (fig 2A and B), and in two genomic regions surrounding the RUNX1 and GAPDH positive control genes (fig 2C and D).
Figure 3. Western blot analysis using the Diagenode antibody directed against CHD7 Whole cell extracts from HeLa (lane 1) and 293T cells (lane 2) were analysed by Western blot using the Diagenode antibody against CHD7 (Cat. No. C15410340) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.
Figure 4. Immunoprecipitation using the Diagenode antibody directed against CHD7 Immunoprecipitation was performed on whole cell extracts from HeLa cells using 6 μg of the Diagenode antibody against CHD7 (cat. No. C15410340, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CHD1 protein was detected by western blot with the CHD1 antibody diluted 1:100.
WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.
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