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CBFb Antibody - ChIP-seq Grade (sample size)

Catalog Number
Format
Price
C15310002-20
20 µl
$95.00
  Bulk order
Other format



Alternative names: PEBP2B, CBF-beta, PEA2-beta, PEB2-beta

Polyclonal antibody raised in rabbit against human CBFb (core-binding factor, beta subunit) using two KLH-conjugated synthetic peptides containing sequences from the central region of the protein.

LotA1329-001
Concentrationnot determined
Species reactivityHuman
TypePolyclonal
PurityWhole antiserum
HostRabbit
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ChIP/ChIP-seq * 4 μl/ChIP Fig 1, 2
ELISA 1:500 Fig 3

* Please note that the optimal antibody amount per ChIP should be determined by the end-user. We recommend testing 1-10 μl per IP.

  • Validation Data

    CBFb Antibody ChIP Grade

    Figure 1. ChIP results obtained with the Diagenode antibody directed against CBFb
    ChIP assays were performed using SKNO-1 cells, the Diagenode antibody against CBFb (Cat. No. C15310002) and optimized primer pairs for qPCR. Sheared chromatin from 1.25 million cells and 4 μl of antibody were used per ChIP experiment. QPCR was performed using primers specific for the FUT7, OGG1, NFE2, and SPI1 genes. Figure 1 shows the relative occupancy, calculated as the ratio + control/background for which the MYOG gene was used.

    CBFb Antibody ChIP-seq Grade

    CBFb Antibody for ChIP-seq

    CBFb Antibody for ChIP-seq assay

    CBFb Antibody validated in ChIP-seq

    Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against CBFb
    ChIP was performed as described above. The IP’d DNA from 6 ChIP’s was pooled and analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 32 bp tags were aligned to the human reference genome (hg18) using the ELAND algorithm. Figure 2 shows the results of the complete chromosome 3 and three genomic regions region surrounding the OGG1, FUT7 and NFE2 genes, respectively. The position of the PCR amplicon is indicated with an arrow.

    CBFb Antibody ELISA Validation

    Figure 3. Determination of the antibody titer
    To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human CBFb (Cat. No. C15310002). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:8,800.

  • Target Description

    CBFb (UniProtKB/Swiss-Prot entry Q13951) represents the beta subunit of a heterodimeric core-binding transcription factor belonging to the PEBP2/CBF transcription factor family. These transcription factors regulate a host of genes specific to haematopoiesis (e.g. RUNX1) and osteogenesis (e.g. RUNX2). The beta subunit is the regulatory subunit which allosterically enhances the activity of the DNA binding alpha subunit as the complex binds to the core site of various enhancers and promoters. CBFb can be involved in a chromosomal rearrangement of chromosome 16 (inv(16)(p13q22)) which produces a fusion protein consisting of the N terminus of CBFb and the C-terminal portion of MYH11. This chromosomal rearrangement is associated with acute myeloid leukaemia of the M4Eo subtype.

  •  Applications
  •  Documents
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
    Download
    Datasheet CBFb C15310002 DATASHEET
    Datasheet description
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  •  Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: CBFb Antibody - ChIP-seq Grade (sample size) (Diagenode Cat# C15310002-20 Lot# A1329-001). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    ERG and FLI1 binding sites demarcate targets for aberrant epigenetic regulation by AML1-ETO in acute myeloid leukemia.
    Martens JH, Mandoli A, Simmer F, Wierenga BJ, Saeed S, Singh AA, Altucci L, Vellenga E, Stunnenberg HG
    ERG and FLI1 are closely related members of the ETS family of transcription factors and have been identified as essential factors for the function and maintenance of normal hematopoietic stem cells. Here, genome-wide analysis revealed that both ERG and FLI1 occupy similar genomic regions as AML1-ETO in t(8;21) AMLs ...

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