Figure 1. Western blot analysis using the Diagenode antibody directed against C2C2
Western blot was performed on protein extracts from U2OS cells (lane 1) and on extracts from U2OS cells spiked with 50 ng of the immunogen (Lsh C2C2 recombinant protein, lane 2) using the Diagenode antibody against C2C2 (Cat. No. C15310267), diluted 1:10,000 in PBS-T containing 3% NFDM. The marker is shown on the left, the position of the C2C2 protein is indicated on the right. It’s unclear what the higher MW signal represents but since it is absent in the lane without the spiked protein, it might be a protein aggregate.