Diagenode

ac4C Antibody (sample size)

Catalog Number
Format
Price
C15200252-10
10 µg
$100.00
  Bulk order
Other format



Monoclonal antibody raised in mouse against 4-acetylcytosine (ac4C) conjugated to KLH.

Lot001
Concentration0.7 µg/µl
Species reactivityHuman, other (wide range): positive.
TypeMonoclonal
PurityProtein G purified monoclonal antibody
HostMouse
Storage ConditionsStore at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.
Storage BufferPBS containing 0.05% sodium azide
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
RIP* 2 µg per IP Fig 1
ELISA 1:1,000 Fig 2

*Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-10 µg per IP.

  • Validation data

    Figure 1. RNA immunoprecipitation using the Diagenode monoclonal antibody directed against ac4C
    RNA immunoprecipitation (RIP) was performed on 40 µg total RNA isolated from HeLa cells using the Diagenode antibody against ac4C (cat. No. C15200252). The RNA was spiked with an in vitro produced RNA molecule containing ac4C nucleotides as well as an unmodified control RNA (300 ng each). A titration of the antibody consisting of 1, 2, 5 and 10 µg per RIP experiment was analysed. IgG as well as an antibody against m6A (C15410208) (both 2 µg/IP) were used as negative IP controls. QRT-PCR was performed with primers specific for the modified and unmodified RNA molecules. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

    Figure 2. Cross reactivity of the Diagenode monoclonal antibody directed against ac4C
    To test the specificity an ELISA was performed using a serial dilution of the Diagenode monoclonal antibody against ac4C (cat. No. C15200252). The wells were coated with the ac4C nucleoside as well as the m4C and unmodified Cytosine nucleosides coupled to BSA. Figure 2 shows a high specificity of the antibody for the modification of interest.

  •  Applications
    ELISA
    Enzyme-linked immunosorbent assay. Read more
    RIP
    RIP Read more
  •  Documents
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
    Download
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
    Download
    ac4C antibody DATASHEET
    Download
  •  Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: ac4C Antibody (sample size) (Diagenode Cat# C15200252-10 Lot# 001). Click here to copy to clipboard.

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Events

  • Virtual ChIP workshop - January 18-19, 2022 or January 25-26, 2022
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    Jan 18-Jan 26, 2022
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    Jan 18-Jan 26, 2022
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    Feb 14-Feb 15, 2022
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