Diagenode

1-methyladenosine monoclonal antibody

Catalog Number
Format
Price
C15200235
100 µg
$305.00
  Bulk order

Other names: m1A, 1mA, m1Ado

Monoclonal antibody raised in mouse against 1-methyladenosine (m1A) conjugated to KLH.

Lot004
Concentration1 μg/μl
Species reactivityHuman, mouse, other (wide range): positive.
TypeMonoclonal
PurityProtein G purified monoclonal antibody.
HostMouse
Storage ConditionsStore at -20°C.
Storage BufferPBS containing 50% glycerol, does not contain a preservative.
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
IP 15 μg per IP Fig 1
IF 1:1,000 Fig 2
IHC 1:10,000 Fig 3
  • Validation data

    RIP

    RIP

    Figure 1. Immunoprecipitation using the Diagenode monoclonal antibody directed against m1A
    Immunoprecipitation was performed on 40 µg total RNA isolated from HEK293 cells using 15 µg of the Diagenode monoclonal antibody against m1A (cat. No. C15200235) or with an equal amount of mouse IgG2b, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the m1A antibody (upper right). The lower figure shows the gel image for the input, the negative IgG2b control and the m1A antibody (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.

    IF

    Figure 2. Immunofluorescence using the Diagenode monoclonal antibody directed against m1A
    HeLa cells were stained with the Diagenode monoclonal antibody against m1A (cat. No. C15200235). Cells were fixed with 4% formaldehyde for 20 min at RT, permeabilized with 0.5% Triton X-100 for 5 min at RT and blocked with PBS containing 1% BSA. The cells were immunofluorescently labeled with the m1A antibody (left) diluted 1:1,000 in blocking solution followed by a goat anti-mouse antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with Hoechst33342. A merge of the two stainings is shown on the right.

    IHC

    Figure 3. Immunohistochemistry using the Diagenode monoclonal antibody directed against m1A
    Paraffin-embedded mouse liver tissue was analysed by immunohistochemical analysis using the Diagenode monoclonal antibody against m1A (cat. No. C15200235) diluted 1:10,000 (brown). The slides were counterstained with Hematoxylin (blue).

  •  Applications
    IP
    Immunoprecipitation Read more
    IF
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
    IHC
    Immunohistochemistry Read more
  •  Documents
    1-methyladenosine monoclonal antibody DATASHEET
    Monoclonal antibody raised in mouse against 1-methyladenosine (m1A) conjugated to KLH.
    Download
  •  Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: 1-methyladenosine monoclonal antibody (Diagenode Cat# C15200235 Lot# 004). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

Events

 See all events

Applications

 See all applications



The European Regional Development Fund and Wallonia are investing in your future.

Extension of industrial buildings and new laboratories.



  ABOUT SSL CERTIFICATES

         Site map   |   Contact us   |   Conditions of sales   |   Conditions of purchase   |   Privacy policy   |   Diagenode Diagnostics