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Figure 1. Western blot analysis using the Diagenode antibody directed against HP1α Western blot was performed on nuclear extracts from HeLa cells (20 μg) with the Diagenode antibody against human HP1α (Cat. No. C15410070), diluted 1:1,000 in TBS-Tween containing 5% skimmed milk (Figure 1). The molecular weight marker (in kDa) is shown on the left; the expected location of HP1α, HP1β and HP1γ is indicated on the right. The western blot analysis clearly demonstrates that the HP1α antibody specifically recognizes HP1α and not HP1β and HP1γ.
Figure 2. Immunofluorescence using the Diagenode antibody directed against HP1α HeLa cells were stained with the Diagenode antibody against HP1α (Cat. No. C15410070) and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the HP1α antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.
WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.
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Diagenode offers huge selection of highly sensitive antibodies validated in IF.
Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9
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