HP1? polyclonal antibody

Catalog Number
50 µg/25 µl
  Bulk order

Alternative names: HP1 alpha, CBX5, HP1A

Polyclonal antibody raised in rabbit against human HP1α (Heterochromatin protein 1 homolog alpha), using the full length recombinant GST tagged protein.

Concentration2.0 µg/µl
Species reactivityHuman
PurityProtein G purified
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
Western Blotting 1:1,000 Fig 1
Immunofluorescence 1:500 Fig 2
* Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μg per IP.
  • Validation Data

    Western blot

    Figure 1. Western blot analysis using the Diagenode antibody directed against HP1α
    Western blot was performed on nuclear extracts from HeLa cells (20 μg) with the Diagenode antibody against human HP1α (Cat. No. C15410070), diluted 1:1,000 in TBS-Tween containing 5% skimmed milk (Figure 1). The molecular weight marker (in kDa) is shown on the left; the expected location of HP1α, HP1β and HP1γ is indicated on the right. The western blot analysis clearly demonstrates that the HP1α antibody specifically recognizes HP1α and not HP1β and HP1γ.


    Figure 2. Immunofluorescence using the Diagenode antibody directed against HP1α
    HeLa cells were stained with the Diagenode antibody against HP1α (Cat. No. C15410070) and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the HP1α antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.

  •  実験手法
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
  •  資料
    Datasheet hHP1alpha pAb-070-050 DATASHEET
    Datasheet description
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
  •  Safety sheets
    HP1 Antibody SDS GB en Download
    HP1 Antibody SDS US en Download
    HP1 Antibody SDS BE nl Download
    HP1 Antibody SDS FR fr Download
    HP1 Antibody SDS BE fr Download
    HP1 Antibody SDS ES es Download
    HP1 Antibody SDS DE de Download
    HP1 Antibody SDS JP ja Download
  •  出版物

    How to properly cite this product in your work

    Diagenode strongly recommends using this: HP1? polyclonal antibody (Diagenode Cat# C15410070 Lot# 001). Click here to copy to clipboard.

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