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Figure 1. H3T3pK4me2 antibody ChIP results Chromatin Immunoprecipitation of H3T3pK4me2 antibody. Chromatin from one million formaldehyde cross-linked Hela cells was used with 2 μg of H3T3pK4me2 and 20 μl of magnetic beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and normalized to the input chromatin.
Figure 2. H3T3pK4me2 antibody Immunofluorescence results Immunofluorescence H3T3pK4me2 antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Primary antibody was used at a 1:50 dilution for 1 h at RT. Secondary antibody: Dylight 488 secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H3T3pK4me2 is nuclear and chromosomal. Staining: H3T3pK4me2 is expressed in green while the nuclei and aplpha-tubulin were coexpressed with DAPI (blue) and Dylight 550 (red).
Figure 3. H3T3pK4me2 antibody Western blot results Western Blot of H3T3pK4me2 antibody. 30 μg of NIH-3T3 histone extracts. Primary antibody used at a 1:500 dilution overnight at 4°C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Predicted/Observed size: ~15 kDa. Other band(s): None.
Figure 4. H3T3pK4me2 antibody Dot blot results Dot Blot of H3T3pK4me2 antibody. Load: 1, 10, and 100 picomoles of the different peptides. Primary antibody used at a 1:1,000 dilution for 45 min at 4°C. Secondary antibody: DylightTM488 rabbit secondary antibody at 1:10,000 for 45 min at RT.
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Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9
HeLa cells transfected with a Cas9 expression vector (... Read more
WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.
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