H3T3pK4me2 polyclonal antibody

50 μg
  Bulk order

Polyclonal antibody raised in rabbit against Histone H3 (dimethyl Lys4, p Thr3) using a KLH-conjugated synthetic peptide.

Concentration0.83 μg/μl
Species reactivityHuman, mouse, C. elegans, rat, chicken, Xenopus, Drosophila, plant
PurityAffinity purified
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ChIP 2-5 μg/million cells Figure 1
Immunohistochemistry 1:50
Immunofluorescence 1:50 Figure 2
Western Blotting 1:500 Figure 3
Dot Blotting 1:500 Figure 4
  • Validation Data

    H3T3pK4me2 Antibody ChIP Grade

    Figure 1. H3T3pK4me2 antibody ChIP results
    Chromatin Immunoprecipitation of H3T3pK4me2 antibody. Chromatin from one million formaldehyde cross-linked Hela cells was used with 2 μg of H3T3pK4me2 and 20 μl of magnetic beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and normalized to the input chromatin.

    H3T3pK4me2 Antibody validated in Immunofluorescence

    Figure 2. H3T3pK4me2 antibody Immunofluorescence results
    Immunofluorescence H3T3pK4me2 antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Primary antibody was used at a 1:50 dilution for 1 h at RT. Secondary antibody: Dylight 488 secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H3T3pK4me2 is nuclear and chromosomal. Staining: H3T3pK4me2 is expressed in green while the nuclei and aplpha-tubulin were coexpressed with DAPI (blue) and Dylight 550 (red).

    H3T3pK4me2 Antibody validated in Western Blot

    Figure 3. H3T3pK4me2 antibody Western blot results
    Western Blot of H3T3pK4me2 antibody. 30 μg of NIH-3T3 histone extracts. Primary antibody used at a 1:500 dilution overnight at 4°C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Predicted/Observed size: ~15 kDa. Other band(s): None.

    H3T3pK4me2 Antibody validated in Dot Blot

    Figure 4. H3T3pK4me2 antibody Dot blot results
    Dot Blot of H3T3pK4me2 antibody. Load: 1, 10, and 100 picomoles of the different peptides. Primary antibody used at a 1:1,000 dilution for 45 min at 4°C. Secondary antibody: DylightTM488 rabbit secondary antibody at 1:10,000 for 45 min at RT.

  •  应用
    Immunohistochemistry Read more
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    Dot blotting Read more
    ChIP-qPCR (ab)
    Read more
  •  文档
    Datasheet H3T3pK4me2 C15410278 DATASHEET
    Datasheet description
  •  出版物

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