H3K56me1 polyclonal antibody

50 μg
  Bulk order

Polyclonal antibody raised in rabbit against Histone H3 (monomethyl Lys56), using a KLH-conjugated synthetic peptide.

Concentration1.1 μg/μl
Species reactivityHuman, mouse, C. elegans, rat, chicken, Xenopus, Drosophila, plant
PurityAffinity purified
Storage ConditionsStore at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.
Storage Buffer0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, 30% glycerol.
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ChIP 2-5 μg/million cells
Immunofluorescence 1:20-1:100 Fig 1
Western Blotting 1 μg/mL Fig 2
Dot Blotting 1:20-1:100 Fig 3
  • Validation Data

    H3K56me1 Antibody validated in Immunofluorescence

    Figure 1. Immunofluorescence
    Immunofluorescence using the H3K56me1 antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Primary antibody used at a 1:100 dilution for 1 h at RT. Secondary antibody: Dylight 488 secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H3K56me1 is nuclear and chromosomal. Staining: Histone H3K56me1 is expressed in green, nuclei and alpha-tubulin are counterstained with DAPI (blue) and Dylight 550 (red).

    H3K56me1 Antibody validated in Western Blot

    Figure 2. Western Blot
    Western Blot using the H3K56me1 antibody. 30 µg C. elegans embryo lysate. Primary antibody used at 1 µg/ml overnight at 4°C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Predicted/Observed size: ~15 kDa. Other band(s): None.

    H3K56me1 Antibody validated in Dot Blot

    Figure 3. Dot Blot
    Dot Blot using the H3K56me1 antibody. Lane 1: H3K561ac. Lane 2: H3K56me1. Lane 3: H3K56me2. Lane 4: H3K56me3. Lane 5: H3K56 unmodified. Load: 1, 10, and 100 picomoles of peptide. Primary antibody used at a 1:40 dilution for 45 min at 4°C. Secondary antibody: DylightTM488 rabbit secondary antibody at 1:10,000 for 45 min at RT.

  •  应用
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    Dot blotting Read more
    ChIP-qPCR (ab)
    Read more
  •  文档
    Datasheet H3K56me1 C15410296 DATASHEET
    Datasheet description
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
  •  出版物

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